Has anyone used a good P2Y1 receptor antibody conjugated to 488 or 647? For FACS and cell sorting!! Thanks!!!
Hello! Does anyone have a empty Crispr/Cas9 donor plasmid, or a donor plasmid that contains homology arms for EMX1, AAVS1 or hROSA26?
07 August 2016 3,175 2 View
Hello! I want to sort out mesenchymal and endothelial populations (essentially the junk) from a population of cells in live Acinar Pancreatic tissue. Can anyone give me suggestions on what array...
05 June 2016 8,517 1 View
Hi! Im performing a westren blot, but my immunoblotting isnt going well. The proteins transfer perfectly on the PVDF membrane, but when I incubate the membrane with my primary abs, the signal...
06 July 2014 2,403 13 View
Can someone please tell me how to make a cell lysis buffer without PMSF I have sodium deoxycholate but no PMSF. I also have NP-40. Please tell me an appropriate recipe one which does not include...
06 July 2014 1,844 7 View
Arthritic induction is required by the protein rich in joints.
05 June 2014 5,065 5 View
Our lab studies the effect of BMP7 on pancreatic-progenitor like cells, however we have not studied the effect of this on bone mineralization. Maybe we could collaborate in this regard!
01 January 1970 7,094 2 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...
04 August 2024 4,255 1 View