A good fluorophore conjugated P2Y1 receptor antibody out there?

Has anyone used a good P2Y1 receptor antibody conjugated to 488 or 647? For FACS and cell sorting!! Thanks!!!

07 August 2016 7,698 0 View

Empty Crispr/Cas9 donor plasmid?

Hello! Does anyone have a empty Crispr/Cas9 donor plasmid, or a donor plasmid that contains homology arms for EMX1, AAVS1 or hROSA26?

07 August 2016 3,065 2 View

What is a good set of antibodies for mesenchymal/fibroblast cells and endothelial cells. Considering I want to eliminate these populations in FACS?

Hello! I want to sort out mesenchymal and endothelial populations (essentially the junk) from a population of cells in live Acinar Pancreatic tissue. Can anyone give me suggestions on what array...

05 June 2016 8,429 1 View

Why am I getting overexposure on my immunoblotting?

Hi! Im performing a westren blot, but my immunoblotting isnt going well. The proteins transfer perfectly on the PVDF membrane, but when I incubate the membrane with my primary abs, the signal...

06 July 2014 2,273 13 View

Is it possible to make a cell lysis buffer without PMSF?

Can someone please tell me how to make a cell lysis buffer without PMSF I have sodium deoxycholate but no PMSF. I also have NP-40. Please tell me an appropriate recipe one which does not include...

06 July 2014 1,717 7 View

In animal models, particularly BALB/c mice, how can I induce arthritic induction using collagen type-II?

Arthritic induction is required by the protein rich in joints.

05 June 2014 4,976 5 View

Do anyone here can help us in the field of molecular docking?

We have synthesized various metal complexes with different environment around the metals. Now to see the application of synthesized complexes we need this study also. we are also interested in...

01 March 2021 1,899 2 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

How can we assess applied digital skills and soft skills through online examinations during COVID-19 semester?

The final qualification assessment for major programs is a strict regimen that involves different stages - oral and written exams, final project viva, internal and external review. How are ICT...

16 February 2021 1,762 8 View

An article on the Iranian state's civil liability in the COVID-19 matter needs supervision; Is anyone interested in collaborating?

Hello. An article on the Iranian state's civil liability in the COVID-19 issue has been written in collaboration with a friend and a comparative look at law and biology. The article is ready to be...

15 February 2021 2,906 2 View

Do you know of an informal caregiver of a person who has recently received a bone marrow transplant?

We are conducting a pilot study of an intervention for informal caregivers of persons who have recently received a bone marrow transplant in the US. If you know of anyone who might be interested,...

13 February 2021 9,174 1 View

Which tropical country is the most easy for obtaining research permit (for biological research)?

Dear all, High beurocracy hihly complicate field ecolocial and evolutionary research nowadays. Nearly all tropical countries (maybe all?) requires research permit for performitng research and in...

04 February 2021 3,672 6 View

Why marketers need a forensic understanding of consumers and a creative, agile mindset?

02 February 2021 6,579 10 View

How can an Undergraduate collaborate with Professors globally for research projects?

I am seeking to increase my experience by working on research projects and aiming to present them as papers

01 February 2021 3,800 4 View

Are there well-established collaborative sorting tasks/datasets?

Hi, I am currently working on research on communication behavior in Mixed Reality. I am planning a study, where dyads perform a task, in which they have to collaboratively cluster or sort cards...

31 January 2021 4,979 3 View

What is the difference between human umbilical vein endothelial cells (HUVECs) and bone marrow endothelial cells?

Hello I am new to work on endothelial cells, and I have been reading about them a lot and trying to know the difference between human umbilical vein endothelial cells (HUVECs) and bone marrow...

28 January 2021 8,765 4 View