Nouara, in such kind of work , we need to keep two things very important  in mind  . You will have to concentrate on saline soil conditions and  specific citrus , if you have citrus species , like mandarin , sweet orange , limes/lemon etc , wonderful .  If we ahve acombination saline soils plus tolerant kin dof citrus species growing in those conditions  Your job of developing AMF consortium easy. Unfortunately , citrus  is not considered as crop tolerant to either salinity or sodicity , unless raised on some tolerant citrus rootstocks like cleopatra mandarin /Rangpur lime. But , you try to isolate the AMF diversity from saline soil conditions ( If you are looking at salt tolerant species) , and later inoculate it with host plant citrus ( I do not know , which citrus species , you are looking at ) to see the response of AMF dependence , that way , you will shortlist the AMF species . For example , you have screened to three most important species . Then , study their progressive response study  and their complimentarity to arrive at conclusion about possibility of consortium , though , it is time consuming ...This is how , we have to go about .....

i'm searching for a fungi for a crop of C35, but it's more against drought than salinity

 Response of most of the plants  against drought versus salinity remains  almost teh same , since salinity effect is triggered through water stress induced via osmotic stress . C35 is a citrange , having trifoliate blood , which is considered susceptibility to salinity .  If you have commercial plantation  satsuma mandarin  raised on citranges or any other citrus species  , you can search/screen  AMFs , otherwise try to isolate AMFs from rhizopshere of other citrus species , or even other perennial fruit crops, but saline soil environment is very important ../

so you have to extract the fungal strains of saline soil ?

For drought and salinity I suggest the culturable mycorrhiza which was identified in saline drought conditions in the Thar Desert. 

Piriformospora

From Wikipedia, the free encyclopedia

Piriformospora

Scientific classification

Kingdom:

Fungi

Division:

Basidiomycota

Class:

Agaricomycetes

Order:

Sebacinales

Family:

Sebacinaceae

Genus:

Piriformospora

Species:

Piriformospora indica

Type species

Piriformospora indica

Sav.Verma, Aj.Varma, Rexer, G.Kost & P.Franken (1998)

Piriformospora is a fungal genus of the order Sebacinales. It is a monotypic genus, containing the single endophytic root-colonising speciesPiriformospora indica, discovered from orchid plants in the Thar desert in Rajasthan, India by Prof. Ajit Verma and group, School of Life Sciences, Jawaharlal Nehru University, New Delhi. The fungus has typical pear-shaped chlamydospores, and thus, named as P. indica . Unlikemycorrhizal fungi, which cannot be cultured axenically, P. indica can be easily grown on various substrates. It has been found to promote plant growth during its mutualistic symbiotic relationship with a wide variety of plants.[1] Experiments have shown that P. indica increases theresistance of colonized plants against fungal pathogens.[2] It has also been found in experiments with barley that P. indica-inoculated plants are tolerant to salt stress and more resistant to root pathogens. P. indica-infested roots also show antioxidant capacity. The fungus also induces systemic disease resistance in plants.[3] P. indica was found to require host cell death for proliferation during mutualistic symbiosis in barley.[4] Itsgenome has been sequenced and was published in 2011.[5]

External links[edit]

List of publications on P. indica*[1]

References[edit]

Jump up^ Verma S, Varma A, Rexer KH, Hassel A, Kost G, Sarbhoy A, Bisen P, Bütehorn B, Franken P (1998). "Piriformospora indica, gen. et sp. nov., a new root-colonizing fungus". Mycologia 90 (5): 896–903. doi:10.2307/3761331.

Jump up^ Serfling A, Wirsel SG, Lind V, Deising HB (2007). "Performance of the biocontrol fungus Piriformospora indica on wheat under greenhouse and field conditions". Phytopathology 97 (4): 523–31. doi:10.1094/PHYTO-97-4-0523. PMID 18943293.

Jump up^ Waller F, Achatz B, Baltruschat H, Fodor J, Becker K, Fischer M, Heier T, Hückelhoven R, Neumann C, von Wettstein D, Franken P, Kogel KH (2005). "The endophytic fungus Piriformospora indica reprograms barley to salt-stress tolerance, disease resistance, and higher yield". Proceedings of the National Academy of Sciences USA 102 (38): 13386–91.doi:10.1073/pnas.0504423102. PMC 1224632. PMID 16174735.

Jump up^ Deshmukh S, Hückelhoven R, Schäfer P, Imani J, Sharma M, Weiss M, Waller F, Kogel KH (2006). "The root endophytic fungus Piriformospora indica requires host cell death for proliferation during mutualistic symbiosis with barley". Proceedings of the National Academy of Sciences USA 105 (49): 18450–7. doi:10.1073/pnas.0605697103. PMC 1697795. PMID 17116870.

Jump up^ Zuccaro, A.; Lahrmann, U.; Güldener, U.; Langen, G.; Pfiffi, S.; Biedenkopf, D.; Wong, P.; Samans, B.; Grimm, C.; Basiewicz, M.; Murat, C.; Martin, F.; Kogel, K. H. (2011). Howlett, Barbara Jane, ed. "Endophytic Life Strategies Decoded by Genome and Transcriptome Analyses of the Mutualistic Root Symbiont Piriformospora indica". PLoS Pathogens 7 (10): e1002290.doi:10.1371/journal.ppat.1002290. PMC 3192844. PMID 22022265.

Its always better to use indigenous/native mycorrhizal species than single/commercial inoculums as far as VAMs are concerned

 Dear Ami Lokhandwala, Good point. Kudos to you. P

Thanks everybody

Citrus is considered highly AM-dependent crop . Yes , i do agree with Paul and Ami , to use native isolates of AMs than the commercial formulations. If you have consortium of AMs suiting to citrus , it is  wonderful . Developing any microbial consortium needs so much of time , and more than that , it is the complementarity test that one has to carry out to ensure that component species are synergistically related to each other , duly supported by field  response test  .

Have you got some bibliography or documents about that?

Well said , this is what we have been propagating  , either for mono-inoculation or for developing microbial consortium ,  we need to  exploit upon the native isolates , most preferably  , Dr Mehta. 

Ok thank you, have you got some informations about this in bibliography?

83

What we have been propagating

I have cleared Laith...we can debate on the issue...