A colleague of mine at a Cdn university is looking for a service provider to ID mammals from fecal pellet DNA. Any suggestions?

More Nicole Sukdeo's questions See All

Is there any research paper about integrating mobile application like google classroom in teaching?

This is to support or serve as a guide for my thesis regarding 21st century teaching strategies. Thankyou so much in advance!

07 December 2020 8,440 4 View

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30 September 2020 7,720 2 View

How should the SWAT-CUP objective function results be interpreted?

I am not sure how to interpret the objective function results in SWAT-CUP. I have six observed variables (two streamflow sites, and four soil moisture sites) with R-squared results ranging from...

29 June 2020 3,652 3 View

Is there a way to conduct robust variance estimation (RVE) using the Comprehensive Meta-Analysis (CMA) program?

I've averaged multiple effect sizes within the same study to a single mean effect size per study, however I know this method underestimates the study's standard error and overestimates study...

04 February 2020 4,738 2 View

How do you lyse small cells (10 microns) without disrupting the mitochondria?

We consistently isolate intact/functional mitochondria from liver and heart tissue using manual homogenization (to disrupt the cell membrane) followed by differential centrifugation. I am now...

04 November 2019 9,279 1 View

What impact does burnt vegetation particulates have upon raainfall and local climate -does it reduce rainfall?

I am no longer at an Academic institution so cannot access many research articles such as Langmann B, Duncan B, Texter C, Trentmann J, van der Werf, GR (2009) Vegetation fire emissions and...

02 October 2019 5,352 3 View

What are specific dendritic cells and macrophages markers for mouse adipose tissue??

I will analyse stromal-vascular fraction of adipose tissue by FACS. The panel of antibodies could be F4/80, Cd11b, Cd45.2, Cd11c and Cd301. The macrophages are f4/80 and Cd11b positive, and Cd11c...

07 May 2019 9,687 1 View

Is Bushnell Haas (BH) Medium appropriate for enrichment of hydrocarbon-degrading microorganisms isolated from marine environment?

The composition of the BH medium is as follows: MgSO4 - 0.2g/L CaCl2 - 0.02g/L KH2PO4 - 1g/L K2HPO4 - 1g/L (NH4)2SO4 - 1g/L and FeCl3 - 0.05g/L Thank you!

06 May 2019 4,680 5 View

Can someone share the syntax for splitting a large SPSS file and saving the output files as CSV or XLS?

I am trying to split a file of over 200,000 cases by a site ID. I need to get these files into excel. I do not want to have to split the SPSS file into 280 smaller files then manually save each as...

16 April 2019 2,903 3 View

How to count cells that are both GFP and dapi positive?

I am doing a competition grow assay where GFP+ keratinocytes and mCherry+ kerationcytes are plated together, allowed to grow and passed multiple times. At each passage, cells are plated onto a...

04 September 2018 4,005 5 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Would fecal samples stored at -20C for over a year impact metagenomic analysis?

Dear All, I have access to some rodent fecal samples that have been stored at -20C instead of -80C. Anyone have experience with the impact of stowage condition on comparative metagenomic analysis...

01 March 2021 2,512 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Does different dilution factors affect negatively on NH3 reading ?

I have a sample of treated water sample & asked to obtain ammonia conc. in sample using a machine with 5-25 ppm detection range. However, upon testing with different dilution factors, all...

27 February 2021 8,483 3 View

How to compare 2 samples recruited with different sampling methods?

If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...

26 February 2021 3,526 3 View

Can random sampling have inclusion criteria?

As random sampling means that everyone in the population have an equal chance to be chosen, can it have inclusion criteria? My research participants have been chosen based on the inclusion...

26 February 2021 6,905 10 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Truman P Young

Try one of these authors (ms available on ResearchGate):

Kartzinel, T.R., P.A. Chen, T.C. Coverdale, D.L. Erickson, W.J. Kress, M.L. Kuzmina, D.I. Rubenstein, W. Wang, R.M. Pringle. 2015. DNA metabarcoding illuminates dietary niche partitioning by African large herbivores. PNAS, online early.

Shirzad Fallahi

The above mentioned authors are Ok

Erin L Koen

You could try the Natural Resources DNA Profiling & Forensic Centre (NRDPFC) at Trent University in Ontario.

http://web.nrdpfc.ca/

Annika Keeley

For bats, a lab at Northern Arizona University is providing this service:

https://www.researchgate.net/publication/275643879_Species_from_Feces_brochure

Research Species from Feces brochure