A colleague of mine at a Cdn university is looking for a service provider to ID mammals from fecal pellet DNA. Any suggestions?

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46 kDa band using a p65 (NFkB) antibody on rat tissue? JESS / Western Blot?

Hi guys, I am running WB on JESS (ProteinSimple) targeting p65 (NFkB) and we are, for some reason, detecting only a 46kDa band in the liver tissue. This is the cytosolic portion of the...

16 June 2024 4,759 0 View

What are the latest social justice issue for physician assisted suicide?

I am having trouble narrowing down the social justice framework of Physician Assisted suicide. thank you for your time. Nicole

09 June 2024 5,836 2 View

What method can I use to quantify trans-fatty acids in foods?

I'd like to measure trans fatty acids in industrial foods. What method of analysis would you recommend? I've heard of microwave-assisted extraction techniques for processing samples. My lab only...

30 April 2024 5,269 1 View

What would your recommendation for an EBP be for a student with ASD who is refusing to start assignments, although all are cognitively appropriate?

Student has HFA and is a 15 year old male

30 April 2024 3,887 2 View

How should microbiology specimens be processed in the laboratory to maintain integrity and accuracy?

Upon reading and understanding the article entitled “Tenets of Specimen Management in Diagnostic Microbiology” by Rajeshwar Reddy Kasarla and Laxmi Pathak, it was highlighted that specimen...

10 April 2024 4,653 3 View

How were sample quality, errors, values, and data evaluated during the transition to lithium heparin gel tubes in the clinical laboratory?

I'm eager for insights concerning the impact on sample quality and data integrity in the clinical laboratory.

11 March 2024 4,081 1 View

Can Rasch analysis indicate which items belong to a certain category?

Can Rasch analysis indicate which items belong to a certain category? I am trying to validate a survey about anxiety. The Likert scale is from 1 to 6 for each of the 17 items. My goal is to...

22 February 2024 6,376 3 View

What would be the appropriate research design if the subject of your study is an inanimate object e.g microbial fuel cell?

I keep getting confused that if it's true experimental design, who would answer the pre and post test?

06 February 2024 8,577 1 View

Can I call inanimate objects as participants?

I am conducting an on-going research study and I am evaluating the performance of consortium of bacteria as a biocatalyst in a microbial fuel cell. There's some misunderstanding between me and the...

05 February 2024 8,252 1 View

What method is better than Indirect Ion Selective Electrode when measuring parameters like Sodium, Potassium, and Chloride?

I am looking for other alternative methods to measure the electrolytes aforementioned since other studies claim that Indirect ISE can induce error in its results due to its dilution step.

24 January 2024 7,519 4 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

If we are using snowball sampling technique, how do we justify the true representativeness of the sample statistically? is there any statistical test?

Are there any statistical methods to justify your sampling technique using SPSS or AMOS?

05 August 2024 9,153 4 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

Truman P Young

Try one of these authors (ms available on ResearchGate):

Kartzinel, T.R., P.A. Chen, T.C. Coverdale, D.L. Erickson, W.J. Kress, M.L. Kuzmina, D.I. Rubenstein, W. Wang, R.M. Pringle. 2015. DNA metabarcoding illuminates dietary niche partitioning by African large herbivores. PNAS, online early.

Shirzad Fallahi

The above mentioned authors are Ok

Erin L Koen

You could try the Natural Resources DNA Profiling & Forensic Centre (NRDPFC) at Trent University in Ontario.

http://web.nrdpfc.ca/

Annika Keeley

For bats, a lab at Northern Arizona University is providing this service:

https://www.researchgate.net/publication/275643879_Species_from_Feces_brochure

Research Species from Feces brochure