Who can hep me to analyze WES data for runs of homozygosity?

Hello, we have performed a WES of a single human sample and I am interested to analyse the runs of homozygosity in this sample. Especially, I am interested to unterstand if segmental uniparental...

10 November 2018 7,445 2 View

Has someone experience using Lipofectamine Stem Transfection Reagent for transfection of human induced pulripotent stem cells (iPSC)?

Has someone experience using Lipofectamine Stem Transfection Reagent for transfection of human induced pulripotent stem cells (iPSC)?

06 July 2018 2,129 0 View

Does T7 Endonuclease need overlapping nucleotides?

We want to know if Cas9 in combination with a sgRNA was working and have performed a PCR in combination with a T7 Assay. Unfortunately, we could not detect any cleavage product for several sgRNAs....

11 December 2017 2,477 1 View

Which bioinformatic prediction tool could be used to predict the influence of indel mutations?

Hi everyone, which other prediction tools (in addition to Provean) could be used to predict the Impact of indel mutations? Thanks for your help! Andreas

06 July 2017 5,616 3 View

Is it possible to compare one sample with a control set of samples?

04 May 2017 7,165 2 View

What are the best fluorescence proteins for structured illumination microscopy?

Hello everyone, I want to do SIM with three different proteins in living cells. Therefore, I need three different fluorescence proteins with high photostability and high brightness? Could anyone...

02 March 2017 3,522 6 View

What is the definition of stable cell line?

02 March 2017 5,366 0 View

Is it possible to use two fluorescence protein tags to increase the brigthness?

Hello everyone, I want to do SIM with living cells and therefore I need fluorescence proteins with high brightness and high photostability. Therefore, I want to ask if someone has fused his/her...

02 March 2017 9,698 3 View

Experience with Bmt1 (restriction enzyme)?

Has anyone experiences with the retriction enzyme BmtI? Are there important specific points to know about this restriction enzyme?

09 October 2014 2,679 1 View

Is it necessary to put the QBI SP163 Enhancer directly upstream of the ATG?

I want to increase the expression of my proteins by inserting the QBI SP163 Enhancer (pcDNA4-Max System, Invitrogen) into another vector system. I want to know if I can add a restriction site (via...

04 May 2014 2,963 0 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

I am looking for an antibody to Arc protein for use in immunohistochemistry with rhesus monkey brain tissue?

Looking for a well-validated antibody to detect Arc protein expression in paraformaldehyde fixed brain tissue using immunohistochemistry. Ideally this antibody would have been published on using...

07 February 2021 8,457 1 View

How should I go about evaluating cytoplasmic and nuclear Arginase-1 activity?

Hello! I've stained some bright field Arginase-1 Hematoxylin slides, but I'm having a hard time finding any computed histology predicates for evaluating the intensity of the cytoplasm verses the...

03 February 2021 3,039 1 View

Antibody dilution for IHQ in OCT frozen tissue is more similar to IHC in Paraffin-embedded samples or to Immunocytochemistry?

31 January 2021 8,678 3 View

Any tips on Fab blocking of mouse tissue?

Hi, everyone, I am working on immunostaining of bone marrow and growth plates of mice. And I have to use an mouse monoclonal Ab to do this staining. However, with only secondary goat anti-mouse...

21 January 2021 9,826 3 View

Antigen retrieval for neural cell marker on frozen brain tissue?

Hi, I'm having problems with the Recombinant Anti-MAP2 antibody from Abcam (Recombinant Anti-MAP2 antibody [EPR19691] (ab183830)) antibody on my frozen, 4%PFA perfused, mice brain tissue. I...

10 January 2021 4,002 5 View

How to approach quantification of cytoplasmic immunofluorescent signal in adipocytes?

Any ideas on how to quantify a non-nuclear immunofluorescent signal in adipocytes? I had the idea to do a watershed on inverted image to "locate" the center of the empty space where lipid droplets...

05 January 2021 5,420 1 View

Can you recommend a protocol for immunohistochemistry of mouse brain sections using anti-MAP2?

Current protocol does not give clear images.

04 January 2021 1,614 3 View

I need to know about immunohistochemistry steps?

Immunohistochemistry staining. Tissue Fixation during IHC. Using DAB. blocking step. controls group.

23 December 2020 5,768 2 View

ISO antibody against BFP for FFPE IHC?

Suggestions and recommendations welcome. Any usage/protocol experience would also be helpful/appreciated. Cheers

22 December 2020 599 3 View