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nitrotyrosine is detected
Don't know what was happening with the "lost" question.
A "?" can only be replied to with "??"
Nitroproteome is in study.
Nitrated protrein have new action on cellular and molecular targets.
Peroxinitrite nitrates and aggregates proteins
Hello, we have performed a WES of a single human sample and I am interested to analyse the runs of homozygosity in this sample. Especially, I am interested to unterstand if segmental uniparental...
10 November 2018 7,539 2 View
Has someone experience using Lipofectamine Stem Transfection Reagent for transfection of human induced pulripotent stem cells (iPSC)?
06 July 2018 2,224 0 View
We want to know if Cas9 in combination with a sgRNA was working and have performed a PCR in combination with a T7 Assay. Unfortunately, we could not detect any cleavage product for several sgRNAs....
11 December 2017 2,576 1 View
Hi everyone, which other prediction tools (in addition to Provean) could be used to predict the Impact of indel mutations? Thanks for your help! Andreas
06 July 2017 5,699 3 View
Hi everyone, I have measured the RNA of a single patient with a specific rare mutation using qRT-PCR and I have compared it with a control set of probands. My problem is, that I have only one...
04 May 2017 7,264 2 View
Hello everyone, I want to do SIM with three different proteins in living cells. Therefore, I need three different fluorescence proteins with high photostability and high brightness? Could anyone...
02 March 2017 3,617 6 View
Hi everyone, if I transfect a plasmid with a resistence gene (puromycin) via Lipofectamin (but not with a virus) into eukaryotic cells and select these cells with puromycin over some weeks is the...
02 March 2017 5,470 0 View
Hello everyone, I want to do SIM with living cells and therefore I need fluorescence proteins with high brightness and high photostability. Therefore, I want to ask if someone has fused his/her...
02 March 2017 9,793 3 View
Has anyone experiences with the retriction enzyme BmtI? Are there important specific points to know about this restriction enzyme?
09 October 2014 2,821 1 View
I want to increase the expression of my proteins by inserting the QBI SP163 Enhancer (pcDNA4-Max System, Invitrogen) into another vector system. I want to know if I can add a restriction site (via...
04 May 2014 3,062 0 View
I am doing immunohistochemistry of the c-Fos protein and I have a budget problem. For context, the experiment has been done over 3 years prior to my arrival and I have 40 brains done and 100 left....
29 July 2024 5,961 2 View
Hello everyone, A collaborator sent us his protocol in order to be able to use his immunohistochemistry kits. But one step contains PBS + 0.3% H2O2 + 0.1% sodium azide. Knowing the dangerousness...
23 July 2024 6,314 3 View
We have mice brains that were over-fixed due to old PFA used during perfusion. Thus, the synapses are no longer being labeled by the Synaptophysin (SY38) mAB. which works perfectly every other...
17 July 2024 7,767 3 View
I'm looking to immunostain some brain tissue collected previously. The animal was *not* perfused prior to tissue collection. Will the presence of blood interfere with all staining methods...
14 July 2024 177 4 View
Hi all, I would like to know if it is possible to use Bovine Serum Albumin (BSA) in my blocking solution for immunofluorescence muscle fiber typing. The primary antibodies' host is mouse. The...
07 July 2024 7,967 3 View
In the context of doctoral research, I utilized immunohistochemical (IHC) analysis to quantify the expression levels of the Ki-67 proliferation marker. While the application of this test and...
25 June 2024 7,825 2 View
Hello! I've recently been trying to optimise immunofluorescent staining of mouse meningeal tissue, and I'm having trouble mounting it just because it's so thick. We use prolong to mount and...
05 June 2024 624 2 View
I need to do immunohistochemistry in mouse brain, particularly in neurotensin-cre mouse line.
22 May 2024 3,565 7 View
Hi all, I've processed my own IHC for many years and never run into this problem before, but the last few times I've tried staining, I've had the same issue. I'm wondering if it's a reagent...
08 May 2024 3,979 1 View
I have been using Shandon Sequenza for the last two years with no problems. Lately i have noticed small spots under a microscope that have not been stained by DAB (only hematoxylin as a contrast...
28 April 2024 2,530 1 View