14 Questions 25 Answers 0 Followers
Questions related from Andreas Brodehl
Hello, we have performed a WES of a single human sample and I am interested to analyse the runs of homozygosity in this sample. Especially, I am interested to unterstand if segmental uniparental...
11 November 2018 7,511 2 View
Has someone experience using Lipofectamine Stem Transfection Reagent for transfection of human induced pulripotent stem cells (iPSC)?
07 July 2018 2,197 0 View
We want to know if Cas9 in combination with a sgRNA was working and have performed a PCR in combination with a T7 Assay. Unfortunately, we could not detect any cleavage product for several sgRNAs....
12 December 2017 2,548 1 View
Hi everyone, which other prediction tools (in addition to Provean) could be used to predict the Impact of indel mutations? Thanks for your help! Andreas
07 July 2017 5,679 3 View
Hi everyone, I have measured the RNA of a single patient with a specific rare mutation using qRT-PCR and I have compared it with a control set of probands. My problem is, that I have only one...
05 May 2017 7,235 2 View
Hi everyone, if I transfect a plasmid with a resistence gene (puromycin) via Lipofectamin (but not with a virus) into eukaryotic cells and select these cells with puromycin over some weeks is the...
03 March 2017 5,429 0 View
Hello everyone, I want to do SIM with living cells and therefore I need fluorescence proteins with high brightness and high photostability. Therefore, I want to ask if someone has fused his/her...
03 March 2017 9,763 3 View
Hello everyone, I want to do SIM with three different proteins in living cells. Therefore, I need three different fluorescence proteins with high photostability and high brightness? Could anyone...
03 March 2017 3,591 6 View
Has anyone experiences with the retriction enzyme BmtI? Are there important specific points to know about this restriction enzyme?
10 October 2014 2,788 1 View
I want to increase the expression of my proteins by inserting the QBI SP163 Enhancer (pcDNA4-Max System, Invitrogen) into another vector system. I want to know if I can add a restriction site (via...
05 May 2014 3,037 0 View
I am planing BiFC assays and before I want to start I have some general questions: What is the strongest BiFC system? At the moment I could not say anything about the strength of my...
05 May 2014 4,048 3 View
What are your transfection efficiencies?
11 November 2013 5,344 3 View
Does anyone know any special program to analyze 3D staples of cells (detected with an 510 ... Zeiss CLSM)? Imaris is to expensive. Has anyone a freeware suggestion. It would be great if this...
11 November 2013 2,350 1 View
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10 October 2013 467 3 View
