I am facing a lot of problems with 3T3-L1 differentiation since last year. I am trying to differentiate them in 96 well plate at 70% confluency using this protocol by Lee et al, 2015 (I have tried various protocols earlier, but non of them worked well. I am following the current one because a senior followed this protocol and she was successful in differentiating the cells and even had proper ORO data from it. She has quit her Ph.D. So, I can't contact her anymore for help). The cells are still proliferating, cell growth hasn't been arrested. Hence, the cell layer is peeling off on Day 5 after induction. Can anyone help me?