What is the role of Current vs. time in galvanostatic deposition of Platinum?

What is the role of current vs. time in galvanostatic deposition of Platinum? Is it possible to accelerate the deposition rate from a very dilute plating solution, by increasing the applied...

03 April 2016 6,683 6 View

How can one make a sputtered gold layer negatively charged?

If a thin gold layer (50 nm) is sputter coated on a wafer, is it possible to make this Au layer negatively charged, for example by adsorbing a negatively charged specie? Can we citrate for this...

31 December 2014 8,144 3 View

How does metal density influence the quality of a sputtered thin layer?

In the case of sputter coating, if we switch from Platinum (density 21.45 g/cm3) to Copper (density 8.96 g/cm3) for the same thickness of 20 nm, how the metal density difference will affect the...

09 October 2014 2,506 5 View

How to enhance adhesion between sputtered Au and Pt thin films?

I have noticed poor adhesion between sputtered Au and Pt thin films. Why this is so, and is there a way to enhance this adhesion?

09 October 2014 9,345 14 View

Is graphite a good material to serve as working electrode?

Can I use colloidal graphite coating as working electrode for the electropolymerization of conductive polymers? e.g. PANI, PEDOT, PPy.

08 September 2014 5,953 4 View

Is an Ag/AgCl Reference Electrode an issue?

08 September 2014 4,188 15 View

How can I remove a sputtered Ag or Au layer from polycarbonate?

I wish to know the method and materials required to remove sputtered Ag or Au layer from the surface of a polycarbonate membrane used for the preparation of nanowires.

07 August 2014 1,738 5 View

Why is a white colored matter produced as soon as Ag/AgCl reference electrode is dipped into a AgNO3 solution?

While trying to deposit Ag, as soon as I dip an Ag/AgCl reference electrode into a Ag deposition solution of 0.2M AgNO3 + H3BO3 + HNO3, white colored matter starts to produce from the interface of...

07 August 2014 724 27 View

Is there a standard way to determine if a metal can be electrochemically deposited on another?

06 July 2014 7,326 12 View

Why I am not able to have reversible CV of potassium ferricynaide/ferrocyanide redox couple?

06 July 2014 1,589 26 View

Anyone that can help with Maxquant TMT16plex which is missing?

Hi I am very new to proteomics analysis. I have got raw files from core facility running thermofisher TMT 16plex 4 hours. I cannot find on maxquant the option TMT16Plex there is only up to 11...

25 February 2021 1,675 3 View

How to manage western blot for proteomics data validation?

Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...

23 February 2021 6,671 4 View

Unable to detect a truncated protein variant, but I can observe a truncated cDNA from Sanger?

Hi, so basically... I am trying to mimic a clinically observed condition wherein a frame-shift mutation happens only at one allele of the patients, giving rise to a truncated variant which evades...

17 February 2021 1,130 6 View

Is it possible to remove the cells from a tissue and keep only the extracellular matrix for proteomics?

Hello! I would like to know if there is a way to flush/remove all the cells out of a tissue and keep only the extracellular matrix, in order to analyse its composition using e.g. proteomics....

14 February 2021 1,602 4 View

Apart from MOFA what other software is available for analysis of a multi-omic study?

Study bringing together genomics, proteomics etc together

02 February 2021 2,856 2 View

How can I reduce run-to-run variation in TMT MS experiments?

I planned an experimental setup using 10-plex TMT with 9 conditions and 1 bridge channel to compare 5 replicates. But since I experienced some run-to-run variation, I was wondering if a better...

31 January 2021 3,405 3 View

Where can I purchase NanoLC emitter fused silica picoTip?

Hi, I'm working with NanoLC/MS for both Metabolomics and Proteomics. We usually make our nano columns using emitters from NewObjective. Unfortunately New Objective, due to the pandemic, is now...

10 January 2021 1,176 4 View

An optimal protocol of EVs isolation for proteomics?

Hi everyone, I am starting a project in analyzing EVs proteomics in cancers. However, the most published articles I have found stated only brief isolation protocols that could not fully be...

17 December 2020 7,600 2 View

Infected plant protein lysate washing out purified GST-tagged protein on agarose resin?

Hi, I am trying to screen infected plant proteomes for targets of a secreted pathogen protein. I have purified the pathogen protein using a GST-tag on an agarose resin. I am using the Pierce...

23 November 2020 7,009 3 View

Dataset: Orbitrap velos CID MS/MS spectra ?

Hi, I want the MS2-CID spectra from orbitrap velos mass spectrometry of organism: human (Mod: C+57) for testing my peptide identification algorithm developed. Large set of orbitrap velos dataset...

16 November 2020 8,261 1 View