Gina Chaput

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Questions related from Gina Chaput

“3′ single end mRNA Seq” or “Tag-Seq” - how low of an RIN score will be successful?

I have two sites that have different claims in their ability to have succesful cDNA libraries from RNA with RIN scores: 1) UC Davis...

23 June 2020 4,263 3 View

How to interpret Agilent Bioanalyzer 2100 results with high RNA concentrations?

I submitted my plant RNA samples to be run on an Agilent Bioanalyzer 2100 and received back the results. Most of the extraction kits I tested were good in quantity (ranging from 40-270ng/uL) as...

23 October 2019 5,578 5 View

Biosafety cabinet for RNA extractions necessary?

I am extracting RNA from milled plant material (BSL1) for downstream RNAseq. If possible, it would be great not to take over the only biosafety cabinet in the lab for RNase free space for weeks...

12 September 2019 8,900 4 View

How can I convert a fasta file OR sequential phylip to an interleaved phylip file in R?

I have been trying to convert my fasta file to an interleaved phylip file in R for a phylogeny project. Currently I have been using the package phylotools, starting with function read.fasta()...

29 July 2018 2,066 0 View