How important is it to use 2x YT rather than regular LB, while growing bacteria to be infected with M13 bacteriophage for Phage Display?
E. coli will grow to a somewhat higher density in 2x YT than it does in LB, but I routinely used LB for growing M13.
2X YT medium contains double the concentration of yeast extract compared to typical LB medium. It is useful for the growth and maintenance of high density of cells for a longer growth period. It contains 16 g/L tryptone, 10 g/L yeast extract and 5 g/L NaCl.
I agree with Michael and Kirstin. I also used LB for growing M13 bacteriophage
This is a list of paper related to the propagation of M13 for different purposes and different scales.
I hope it can help you to find out the best approach to propagate your M13 stocks.
[1] Jurač, K., Nabergoj, D., & Podgornik, A. (2019). Bacteriophage Production Processes. Applied Microbiology and Biotechnology, 103(2), 685–694. https://doi.org/10.1007/s00253-018-9527-y
[2] Harwood, A. J., & Tomley, F. M. (2003). M13 Phage Growth and Single-Stranded DNA Preparation. In Basic DNA and RNA Protocols. https://doi.org/10.1385/0-89603-402-x:359
[3] Smits, M. A., Simons, G., Konings, R. N. H., & Schoenmakers, J. G. G. (1978). Expression of Bacteriophage M13 DNA in vivo. Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 521(1), 27–44. https://doi.org/10.1016/0005-2787(78)90246-0
[4] Kick, B., Behler, K. L., Severin, T. S., & Weuster-Botz, D. (2017). Chemostat Studies of Bacteriophage M13 Infected Escherichia coli JM109 for cCntinuous ssDNA Production. Journal of Biotechnology, 258, 92–100. https://doi.org/10.1016/j.jbiotec.2017.06.409
[5] Kick, B., Hensler, S., Praetorius, F., Dietz, H., & Weuster-Botz, D. (2017). Specific Growth Rate and Multiplicity of Infection Affect High-Cell-Density Fermentation with Bacteriophage M13 for ssDNA Production. Biotechnology and Bioengineering, 114(4), 777–784. https://doi.org/10.1002/bit.26200
[6] Grieco, S.-H. H., Wong, A. Y. K., Dunbar, W. S., MacGillivray, R. T. A., & Curtis, S. B. (2012). Optimization of Fermentation Parameters in Phage Production Using Response Surface Methodology. Journal of Industrial Microbiology & Biotechnology, 39(10), 1515–1522. https://doi.org/10.1007/s10295-012-1148-3
[7] Warner, C. M., Barker, N., Lee, S. W., & Perkins, E. J. (2014). M13 Bacteriophage Production for Large-Scale Applications. Bioprocess and Biosystems Engineering, 37(10), 2067–2072. https://doi.org/10.1007/s00449-014-1184-7
[8] Machida, K., & Imataka, H. (2015). Production Methods for Viral Particles. Biotechnology Letters, 37(4), 753–760. https://doi.org/10.1007/s10529-014-1741-9
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