Best way to draw COLONY output / pedigrees?

Hi, I am using the sibship analysis software COLONY 2.0.6.6 (most recent version) on about 150 individuals offspring, with some of them as potential fathers and mothers. COLONY provides a lot...

02 February 2021 6,910 2 View

Is frontiers reliable for publishing research in the field of hydrology?

Over the last few years I followed the debate about the frontiers journals. I noticed that the reception seems to vary wildly depending on the field. While some parts of the frontiers group seem...

19 October 2020 4,022 6 View

How is ambidexterity measured on an individual level?

Hello, referring to the concept of Ambidexterity within the business-world/ organizations (e. g. Gibson and Birkinshaw 2004, March 1991, O'Reilly and Tushmann 2013): If one understands...

12 October 2020 5,530 3 View

Have Quantum Cascade Lasers been used to excite phonons (e.g. in solid-state samples)?

I am aware of many femtosecond laser based approaches to excite phonon modes e.g. in crystals and minerals. Quantum Cascade Lasers (QCLs) seem like an interesting alternative, especially since...

27 May 2020 5,464 3 View

What is the deep philosophy behind Uncertainty Principle?

We have learned in QM the famous U. Principle which is probably the most important thing in this branch. We also have learned that space-time stays together in GR. The problem of measurements...

12 April 2020 1,532 1 View

Fe-57 foil for Mossbauer experiments: Does it rust and how to prevent it from rusting?

I'm about to order some Fe-57 foil from an isotope supplier. However, I worry about oxidation. Does the foil need to be kept in an inert environment during all storage and use? Or do such foils...

18 February 2020 8,789 3 View

Scopus Listed Conference?

Hello everybody, I am looking for scopus listed conferences in Europe in the fields - business management - innovation - entrepreneurship - banking - digitalization Are there any websites where...

12 February 2020 881 8 View

What are promising research topics in the field of network security?

I'm currently working on my proposal for my master's thesis and would like to find out which current research topics in the field of network security are promising/interesting/hot.

27 January 2020 7,651 8 View

Is there a lab that determines plankton sample energy contents?

Dear all, I was wondering if anybody knows of a lab that analyses foreign powderised plankton samples via bomb-calorimetry for energy density? I got some energy values that are one order of...

09 December 2019 6,029 3 View

Which requirements are necessary to generate Carbon Nanotube - NMP slurries for battery applications?

I like to start working in dispersions of carbon nanotubes in NMP for battery applications. Talking to different people, things like NMP-water updtake and dry-room requirements were mentioned. I...

19 November 2019 7,662 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View