16S rRNA: SILVA 99% or 97% OTUs?

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Abhijeet Singh

It is described in detail at https://www.arb-silva.de/projects/ssu-ref-nr/

The purpose of it is just to remove the duplicate or highly similar sequences to make the database non-redundant/small.

Yann Gager

Thank you for your answer

Patryk Krauze

Hey Yann,

I assume you know this, but the scientific community is moving towards the analysis of amplicon sequence variants (ASV) instead of OTUs. With that, it is not necessary that you have to decide between the different thresholds.

https://www.nature.com/articles/ismej2017119

Best

Patryk

Dear Patryk,

thanks a lot for your answer, it is really interesting.

Best greetings,

Yann