16s and 23s rDNA sequencing results

01 January 1970 1 9K Report

Hi everybody,

I have bacterial isolates and after the isolation of the DNA, the amplification of the DNA was done for 2.4 kb (approximately) fragment encompassing the 16S, the spacer region and part of the 23S rDNA was done using universal primers Up68 and Up69. then the PCR products were sequenced. my question is about how to merge the sequence of UP68 to the up69 in order to identify the isolates using NCBI?

Ebrahim Elkhtab

After deep research and advice from a friend, I used the bioedit tool to do this job.

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Science method

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