How can I prevent me Elisa sample results from running high compared to specification and prevent plates from failing?

01 February 2015 1,405 4 View

Why is SDS PAGE used for SOS response research?

11 December 2014 1,402 2 View

Can someone suggest a test method to find how much acetone should be mixed with water to get good results?

I have a reagent where I will be mixing it with acetone + water. How do I come up with proportion for it?

09 October 2013 1,483 2 View

Can I use acetone to store my reagents?

As acetone is soluble with many reagents it seems to be working with mine. But I am not sure if it is a good idea to use acetone instead of H2O in reagents?

09 October 2013 286 4 View

Is there a way to identify the stability of the reagent ?

We dont have HPLC in our lab. Some chemical analysis will be of big help. I added some reducing agents to see if it stops oxidizing my reagent. Now I will have to wait a month to see some...

09 October 2013 8,197 8 View

What would the product of these two compounds be?

Pyrogallol and Tungstocilic Acid (TSA). I get a yellow color and was wondering what reaction is causing this.

09 October 2013 6,081 3 View

Test method to detect component from the reagent - can anyone help?

Is there a test I can go with? Please give some reasonable test methods as our lab doesn't have lot of equipment's other than the plate reader. How can I evaluate the concentration of metol from...

09 October 2013 7,038 3 View

Reagent Stability?

I am currently having an issue. My reagent shows some slight coloration overtime. As soon as we see coloration I see my test failing most often. I think the reagent loses its enhancing potency...

08 September 2013 1,629 3 View

Reagent stabilizer

My reagent shows slight color change in six months ( Colorless to slight yellow). It does affect my test runs after 6 months giving me low signal. But when I checked its ph ,redox it is same as...

08 September 2013 6,421 3 View

How do I determine the concentration of Silver from a AgNO3 sample with a spectrophotometer?

Anyother method to determine the loss of silver ions overtime and find the concentration of Silver? Note : when I tried it the sample shows reduction. The difference between the 2 min and 5 min...

03 April 2013 7,331 9 View

Can polymer concentrations be calculated based on the number average molecular weight?

Hello, I spin-cast two different polymers onto wafers and measure their height as a function of initial concentration. For polymer A, I have the weight-average molecular weight (Mw) as well as...

28 February 2021 7,882 2 View

How to resolve Gaussian error termination " Error termination via Lnk1e in F:\G16W\l103.exe" for TS(QST3)?

When I run the job for TS(QST3) in gaseous phase, it was completed without any error. But when I add water in CPCM model it terminated with an error: Inconsistency: ModMin= 2 Eigenvalue=...

21 February 2021 3,198 2 View

Using the Pfaffl method: Is it possible that the ratio of the calibrator does not equal 1?

Hi all, I have a question regarding calculations using the Pfaffl method. I have 3 groups per experiment: 1 control group (calibrator) and 2 treatment groups with 6 to 7 biological repeats per...

19 February 2021 8,351 2 View

How to calculate radial distribution function (RDF) of a complex from Ab initio MD simulation trajectory?

Hi all, Is there any software or webserver to read the molecular dynamics (MD) simulation trajectory which obtained from Ab initio MD simulation to calculate the atom-atom radial distribution...

25 January 2021 9,474 3 View

Solving diffusion- reversible chemical reaction equation: How to solve both terms numerically?

I am using finite difference method to discretize the parabolic equation (explicit method), but confused about the chemical reaction term. Solving the generic equation for i specie involves a...

24 January 2021 1,149 18 View

Doubt in DFT geometry preparation?

Hi all, I am trying to perform some ab inito DFT calculations using cp2k code. I have a unit cell of 120 atoms and would like to know how to refine or reduce the number of atoms in the unit cell...

20 January 2021 6,024 3 View

Why it is required to optimize a unit cell generated from cif lattice parameters and atomic coordinates during ab initio solid state calculations?

A cif file represents a unit cell with minimum energy. My question is, in the start of any ab initio calculation for unit cell generated from the cif file, why we are advised to optimize cell...

19 January 2021 9,883 3 View

Is it possible to calculate entalphy of LiBr/H2O or another solution if we know correlation of specific heat without using any software?

Is it possible to calculate entalphy of LiBr/H2O or another solution if we know correlation of specific heat without using any software?

17 January 2021 5,522 3 View

What is the best way to calculate electrical conductivity of semiconductors and the same system with a layer of oxide using Quantum Espresso?

I have tried BoltzTrap, and EPW but I'm not sure whether Boltzmann Transport Equation is a right approach for a complex system (semi cond. + an Oxide layer or even the semiconductor itself). Also...

11 January 2021 4,597 4 View

How to calculate IR and RAMAN spectra form Born charge ?

I am using crystal 17 for my solid state ab initio calculations. For some reason the program is not able to produce the Raman spectra but have generated born charges. Is there any way to produce...

11 January 2021 2,317 2 View