How can I prevent me Elisa sample results from running high compared to specification and prevent plates from failing?

01 February 2015 1,405 4 View

Why is SDS PAGE used for SOS response research?

11 December 2014 1,402 2 View

1:25000?

11 December 2014 3,612 6 View

Can someone suggest a test method to find how much acetone should be mixed with water to get good results?

I have a reagent where I will be mixing it with acetone + water. How do I come up with proportion for it?

09 October 2013 1,483 2 View

Can I use acetone to store my reagents?

As acetone is soluble with many reagents it seems to be working with mine. But I am not sure if it is a good idea to use acetone instead of H2O in reagents?

09 October 2013 286 4 View

Is there a way to identify the stability of the reagent ?

We dont have HPLC in our lab. Some chemical analysis will be of big help. I added some reducing agents to see if it stops oxidizing my reagent. Now I will have to wait a month to see some...

09 October 2013 8,197 8 View

What would the product of these two compounds be?

Pyrogallol and Tungstocilic Acid (TSA). I get a yellow color and was wondering what reaction is causing this.

09 October 2013 6,081 3 View

Reagent Stability?

I am currently having an issue. My reagent shows some slight coloration overtime. As soon as we see coloration I see my test failing most often. I think the reagent loses its enhancing potency...

08 September 2013 1,629 3 View

Reagent stabilizer

My reagent shows slight color change in six months ( Colorless to slight yellow). It does affect my test runs after 6 months giving me low signal. But when I checked its ph ,redox it is same as...

08 September 2013 6,421 3 View

How do I determine the concentration of Silver from a AgNO3 sample with a spectrophotometer?

Anyother method to determine the loss of silver ions overtime and find the concentration of Silver? Note : when I tried it the sample shows reduction. The difference between the 2 min and 5 min...

03 April 2013 7,331 9 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

How can we theoretically calculate Elemental analysis(percentage) of atoms in covalent organic framework?

For example in one research article the Elemental analysis values of COF-TpPa-1 ( Triformylphloroglucinol (Tp) (63 mg, 0.3 mmol), Paraphenylenediamine (Pa-1) (48 mg, 0.45 mmol) are as follows...

24 February 2021 1,265 6 View