I want to run RNA samples on gel electrophoresis. Many protocols suggest to run at 10 V/cm. Does that simply mean I set 10 for the voltage (which we normally use 120 V for DNA electrophoresis)?
More exactly, it is the distance between the electrodes, not the length of the gel that should be taken into account to determine the voltage at which you will run your electrophoresis (Usually, the gel length and the distance between the electrodes are not very different).
10 Volts per cm length of your gel. E.g. if your gel is 12 cm long, then run your samples at 120 V.
More exactly, it is the distance between the electrodes, not the length of the gel that should be taken into account to determine the voltage at which you will run your electrophoresis (Usually, the gel length and the distance between the electrodes are not very different).
Hi
The distance in cm refers to the distance between the electrodes. A volt per centimeter (V/cm) is a decimal multiple of the SI derived unit of the strength of the electric field volt per meter.
Hello
The best voltage applied on agarose gel is 4–10 V/cm and the distance represents length between cathod and anode
Hello,
Note that this 5V/cm is the recommended voltage for running TBE and TAE buffers. The LAB buffer (https://openwetware.org/wiki/LAB_Media) has a lower conductivity and hence less heat is generated at the same voltage (the heat causes lower band resolution because it affects the agarose gel structure, and you can even melt your gel if the generated heat is too high): this means you can crank up voltage to 30 V/cm using LAB, which also has much better resolution with small bands (
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