Ruben Alvarez-Fernandez

6 Questions 91 Answers 0 Followers

Questions related from Ruben Alvarez-Fernandez

In a bicistronic transcript in mammalian cells, is the distance from the STOP of the first coding region to the IRES crucial to the second coding?

Hello all, Long question, I know! In a nutshell, I have a construct that looks like: promoterCMV::Coding1::STOPcodon::untranslated-150bp::IRES::Coding2. The end of the translated Coding1 is...

02 February 2019 5,869 7 View

Cell permeability of ribonucleoside-vanadyl complexes?

Dear all, Does anyone know if these RNAse inhibitors are (yeast)cell-permeable? I was thinking of using them in fission yeast in addition to SuperRNAseIn (Thermo), because the latter does not...

05 May 2016 3,170 3 View

How is primer annealing and optimal concentration influenced by 5' dangling ends in PCR?

Dear all, Just wondering about the effect of very long 5' dangling ends (>60bp) on PCR in the first few cycles. An obvious thing is that after extending them once, they are effectively not...

02 February 2016 9,042 11 View

Home-made recipes for molecular biology, anyone interested?

Hi all, I just thought that I could share a few home-made recipes for well-established commercial kits. I have tried them with my hands and they work pretty well. - RNA extraction and clean-up,...

04 April 2015 5,196 8 View

Does anyone have the pBDGAL4 plasmid?

This plasmid is an old one for yeast two-hybrid work, but I am trying to find it to set up a practical. It has been discontinued from the original source, Agilent, and I have not found it in...

09 September 2014 9,752 6 View

Can anyone provide this vector - pBGWFS7?

I am after the vector above, a Gateway with a eGFP::GUS tag. Would anyone kindly provide it? Thank you.

04 April 2014 1,867 2 View