Do you know any other ways to get inexpensive RADSeq oligonucleotides?
I have never done a qPCR experiment before so I need help with designing one. The attached is the layout of a 48-well plate that I have designed. I am measuring mRNA expression during a 48-hour...
08 September 2013 625 6 View
Does anyone have SDS based DNA isolation protocol from Aedes aegypti mosquito larvae or larvae of any related organisms? I have to isolate DNA from freshly emerged mosquito larvae and older. I...
08 September 2013 5,538 1 View
I need to size dsRNA on agarose gel. I am wondering if I can use a DNA ladder. I was looking at the dsRNA ladder from NEB but the largest band is only 500 bp. However, my gene is supposed to be...
08 September 2013 1,913 2 View
Hello, Is that a must that I digest my RNA sample with DNase during purification? I am using Qiagen RNeasy protect mini kit. Further, I will be using SYBR green qPCR kit. My RNA target does...
05 June 2013 9,298 4 View
I just ran a and SDS PAGE with 12% resolving gel and 4% stacking gel. The problem is that as soon as I turned on the electricity (180V), the samples moved down the stacking gel in a wiggly manner....
05 June 2013 1,633 1 View
I want to run RNA samples on gel electrophoresis. Many protocols suggest to run at 10 V/cm. Does that simply mean I set 10 for the voltage (which we normally use 120 V for DNA electrophoresis)?
05 June 2013 4,173 6 View
I am designing an RNAi experiment. I want to observe gene expression in different samples. Would you recommend Taqman or SYBR green assay? I am convinced that SYBR Green assay would be...
05 June 2013 1,309 57 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....
01 March 2021 3,607 3 View
I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...
27 February 2021 547 3 View
Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...
25 February 2021 6,969 3 View
Hello, I have used the shell function on SpaceClaim to create thin solids for the model I am studying. These are contained within an enclosure for my study. I chose this approach as it avoids the...
24 February 2021 8,856 3 View
I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...
24 February 2021 4,397 3 View
Hi every one Is it possible to define biogas as a fuel in the reformer process(scwg) in Homer software? Biogas fuel is not available in the options thanks
23 February 2021 6,518 3 View
I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...
19 February 2021 7,006 5 View
Remote history of Campylobacter infection with reactive arthritis, and Spitz nevus (UMP). Arthropod exposure in Zika/ChikV endemic area prior to emergence of length dependent, sensory PN with...
18 February 2021 8,719 1 View
I am a research tech in a wildlife genetics group and my PI and I are trying to figure out how/where to put raw reads in a repository. We have capture-seq NGS from Rapid Genomics. The post-doc who...
17 February 2021 7,575 2 View
