Do you know any other ways to get inexpensive RADSeq oligonucleotides?
I have never done a qPCR experiment before so I need help with designing one. The attached is the layout of a 48-well plate that I have designed. I am measuring mRNA expression during a 48-hour...
08 September 2013 713 6 View
Does anyone have SDS based DNA isolation protocol from Aedes aegypti mosquito larvae or larvae of any related organisms? I have to isolate DNA from freshly emerged mosquito larvae and older. I...
08 September 2013 5,640 1 View
I need to size dsRNA on agarose gel. I am wondering if I can use a DNA ladder. I was looking at the dsRNA ladder from NEB but the largest band is only 500 bp. However, my gene is supposed to be...
08 September 2013 2,007 2 View
Hello, Is that a must that I digest my RNA sample with DNase during purification? I am using Qiagen RNeasy protect mini kit. Further, I will be using SYBR green qPCR kit. My RNA target does...
05 June 2013 9,463 4 View
I just ran a and SDS PAGE with 12% resolving gel and 4% stacking gel. The problem is that as soon as I turned on the electricity (180V), the samples moved down the stacking gel in a wiggly manner....
05 June 2013 1,758 1 View
I want to run RNA samples on gel electrophoresis. Many protocols suggest to run at 10 V/cm. Does that simply mean I set 10 for the voltage (which we normally use 120 V for DNA electrophoresis)?
05 June 2013 4,345 6 View
I am designing an RNAi experiment. I want to observe gene expression in different samples. Would you recommend Taqman or SYBR green assay? I am convinced that SYBR Green assay would be...
05 June 2013 1,412 57 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...
06 August 2024 3,130 2 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
Hi all. As a beginner in ChIP-seq experiments, I hope you understand that the following questions might be somewhat basic. I am planning to perform ChIP-seq or MeDIP-seq analysis to investigate...
28 July 2024 6,938 1 View
Gene sequencing related trouble shooting
25 July 2024 4,149 2 View
In running two-dimensional gel electrophoresis on bacterial protein, some spots that appear to match a protein sequence have a significantly more acidic isoelectric point than the calculated pI....
24 July 2024 8,076 3 View
Hello researchers, Sorry for my stupid question. I am learning the QIIME2 workflow for analyzing some 16s amplicon NGS fastq data. I found a very nice paper with data and code public available...
20 July 2024 5,405 2 View
Hello all, I extracted RNA from my samples and performed RIP-seq. After annotating the genomic regions using R, I obtained promoters, exons, introns, and UTRs. Given that my samples consist of...
18 July 2024 1,579 2 View
How is biomass energy essential in the power generation in a country.
08 July 2024 9,477 2 View
