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Questions related from Nanda Aung
Do you know any other ways to get inexpensive RADSeq oligonucleotides?
01 January 2014 1,000 0 View
I need to size dsRNA on agarose gel. I am wondering if I can use a DNA ladder. I was looking at the dsRNA ladder from NEB but the largest band is only 500 bp. However, my gene is supposed to be...
09 September 2013 1,982 2 View
I have never done a qPCR experiment before so I need help with designing one. The attached is the layout of a 48-well plate that I have designed. I am measuring mRNA expression during a 48-hour...
09 September 2013 695 6 View
Does anyone have SDS based DNA isolation protocol from Aedes aegypti mosquito larvae or larvae of any related organisms? I have to isolate DNA from freshly emerged mosquito larvae and older. I...
09 September 2013 5,619 1 View
I just ran a and SDS PAGE with 12% resolving gel and 4% stacking gel. The problem is that as soon as I turned on the electricity (180V), the samples moved down the stacking gel in a wiggly manner....
06 June 2013 1,726 1 View
I want to run RNA samples on gel electrophoresis. Many protocols suggest to run at 10 V/cm. Does that simply mean I set 10 for the voltage (which we normally use 120 V for DNA electrophoresis)?
06 June 2013 4,314 6 View
Hello, Is that a must that I digest my RNA sample with DNase during purification? I am using Qiagen RNeasy protect mini kit. Further, I will be using SYBR green qPCR kit. My RNA target does...
06 June 2013 9,425 4 View
I am designing an RNAi experiment. I want to observe gene expression in different samples. Would you recommend Taqman or SYBR green assay? I am convinced that SYBR Green assay would be...
06 June 2013 1,390 57 View
