An activity in which the body is propelled by moving the legs rapidly. Running is performed at a moderate to rapid pace and should be... | Contact experts in Running to get answers
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Impact of fumigation with phosphine on viability of wheat grains stored for six months at two levels of moisture content, in addition to description of four new records of associat... Article ·...
16 March 2024 7,648 0 View
Hello Everyone, I am planning to do siRNA transfection in PCLS (Precision-cut lung slices) incubated in a culture medium where I want to mix 3 siRNA targets for the same gene to make siRNA pool...
12 March 2024 8,338 0 View
Hello, I have been cultivating RAW264.7 cells from ATCC recently. I culture them in DMEM medium + 10% FBS at a density of 10,000 cells/cm² in a T75 flask with a volume of 10mL. For cell...
28 February 2024 4,438 2 View
I am working with tumor, precancerous and normal tissue from hamster cheekpouches. I am not able to detect the expression of GADPH, b-Actin, Rpl13a and Tubb housekeeping genes with qPCR in normal...
22 February 2024 9,041 7 View
Hi all, I am currently in the exploration of detecting multi-stage cyber attacks. I've encountered challenges regarding the availability and quality of existing benchmark datasets. Many publicly...
18 February 2024 3,034 1 View
We are using 5 different test concentrations of sea urchin ethanolic extracts, and we would like to prepare it using a stock solution of our crude extract with a known concentration. What diluting...
16 February 2024 3,909 0 View
Hi ALL, I am using a pair of primers to amplify a region in my gene of interest from cDNA samples. The cDNA samples are extracted from tissues of mouse of different ages. The gene is known to...
12 February 2024 3,614 4 View
I saw these structures forming in my cell culture. Does anyone know what these are? Have you experienced such a situation before?
05 February 2024 3,736 6 View
In my experiment, I activate a creER mouseline using tamoxifen. The mouse is firstly injected with a cre-dependent virus in the brain, then after several days, tamoxifen is injected i.p. to...
01 February 2024 7,884 1 View
Hi i have estimated an armax model using python sippy library. The estimation gives me two transfer functions H and G. How can I combine them into a single one to predict model output for new...
21 January 2024 1,169 2 View
📷 Chinatsu Yui Wed, Jan 17, 10:44 PM (2 days ago) 📷 📷 to me 📷 Dear Professor. G. Shashikumar I hope this email finds you well. As a lecture coordinator, I am writing to invite you to deliver a...
19 January 2024 5,533 1 View
Hi! I am running a coarse grained simulation with charge regulation to study the charge distribution in a fiber. The system is a coarse grained model with implicit solvent. I fixed the fiber in...
09 January 2024 9,424 0 View
09 January 2024 7,654 0 View
Is there any difference between process time and temperature of cDNA synthesis using common primers (Oligo dT and Random Hexamer) compared with Stem-Loop primer of miRs?
05 January 2024 1,272 2 View
i need to know what have the high demand in future im a hard working individual and have a passionate about future
26 November 2023 1,431 1 View
Iwant to know have anyone any experience about making 1000x sybergreen from it powder?
07 November 2023 1,455 0 View
Hello community, I am presently engaged in the process of genome annotation, specifically focusing on identifying repetitive elements within my plant genome. I have successfully identified both...
30 August 2023 564 0 View
Hi, I want to know how to implement TB-mBj in my DFT calculations in Quantum Espresso. Should I modify my input file or should I change my pseudopotentials? If so where can I find the specific...
25 August 2023 8,388 0 View
i have an adopted scale.do i need to still run EFA and CFA. or there is an alternate approach. Please cite the use of SmartPLS: Ringle, C. M., Wende, S., and Becker, J.-M. 2022. "SmartPLS 4."...
06 August 2023 7,071 4 View
"Statistical Analysis System" (SAS) reservoir performance
31 July 2023 3,770 1 View
I used Biotek Epoch 2 nanoplate reader for quantification of my nucleic acid samples. I do not understand what does the above mentioned statement signify.
27 June 2023 3,389 1 View
I'm having a problem with my current Maxquant files: when comparing to the peptide counts and number of proteins of the same sample, Maxquant finds a lot less than Mascot (about half for...
23 May 2023 5,099 0 View
Hi everyone, I need to transfect 5'ppp-dsRNA (invivoGen) to stimulate RIG-I pathway using Lipofectamine 2000 for Bone marrow-derived macrophage. If you have experience with this, please share your...
18 May 2023 6,596 1 View
Hi Scholars: Hi Schiolars: I obtained my phd degree from The University of Melbourne, Australia in late 2021. Then, I got a job in Taiwan which didn't work out for me. Now, after some...
15 May 2023 4,666 2 View