If I am mixing two siRNAs equally that both have 10 μM concentration to make an siRNA pool. what would be the final concentration of the pool?

12 March 2024 0 8K Report

Hello Everyone,

I am planning to do siRNA transfection in PCLS (Precision-cut lung slices) incubated in a culture medium where I want to mix 3 siRNA targets for the same gene to make siRNA pool but I am puzzled about the final concentration of the pool considering mixing all the siRNAs equally with the same concentration. Also, would you advise regarding the starting concentration of the siRNA (whether it's a pool or single), I am using Lipofectamine® RNAiMAX protocol where they suggested starting with 10 μM as a starting concentration in the attached table.

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