I got 260/280 ratio between 1.8-1.99, Also I got 260/230 ratio around 0.6-0.95 and concentration is around 390-400 ng/ul (RNA dissolved in DNAase/RNAase free water). I want to run rtPCR and eventually do qPCR. Is it suitable/reliable to use this RNA for qPCR?
I encourage you to this new protocol if you work with Eukaryotic Cell:
http://www.nature.com/protocolexchange/protocols/3431
please, Don' forget to leave your comment on the webpage of nature!
I encourage you to apply this new protocol if you work with Eukaryotic Cell:
http://www.nature.com/protocolexchange/protocols/3431
please, Don' forget to leave your comment on the webpage of nature!
If you have enough volume of each sample you can try to precipitate with sodium acetate (and glycogen will also help) and resuspend in a small volume. Precipitation worked for me to improve the ratio BUT the concentration will be lower and that's why you'll need to resuspend in a smaller volume than before and you'll have less rna for the qPCRs...Good luck!
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