Particle size distributions of cross-linked enzymes can be measured by a variety of techniques, including SDS-PAGE, size exclusion chromatography (SEC), SEC coupled with multiangle light scattering (SEC-MALS), analytical ultracentrifugation techniques, dynamic light scattering and other light scattering techniques. The choice of technique will be influenced by the size of the particles and equipment availability.
Covalent crosslinking is usually not reversible. However, there are reversible crosslinking agents, such as those containing disulfide bonds that can be split by reducing agents.