What is the best protocol of sample preparation for SEM analysis of Cyanobacteria?

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Moza Maria Iasmina

Hi! We just started to test some protocols for that....like grown the colony in liquid medium, centrifuged at 1300 for 2 min , obtain a nice pellet and then obtain the image...but we using ESEM! keep in touch :)

Vladimir Dusevich

There are a lot of publications on the web, and it looks like specimen prep protocol for cyanobacteria is pretty straightforward.

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC321274/

http://www.limnetica.com/Limnetica/Limne23/L23a179_Mycroscopy_research_cyanobacteria.pdf

http://www.int-res.com/articles/meps/154/m154p281.pdf

Tarkeshwar Singh

Thank you very much Dr. Vladimir