At other of a naïve cells meaning, What is the probability that there will be activation of a naïve T cell bu an Antigen Presenting Cells (APCs)
Depends on what you mean by activation.
When you have the naive T cell, the APC and the relevant antigen present (in vitro): If measuring activation by secretion of IFN-g, it takes 24-48 hours to see a measurable response (by CBA, ELISA or ELISpot). If measuring CD107a expression, you would see a response within 6 hours. If looking at T cell proliferation as a measure of activation, it takes 5-6 days.
In any case, the time required for activation would depend on a multitude of factors including the reactivity of the donor T cells, immunogenicity of the antigen ( strong antigens need less time), the nature of the antigen (peptides take less time compared to full length proteins), the antigen presenting capability of the APCs and so on.
It depends upon interaction between an APC and naive T cell. In the absence of any "antigen" that is to be presented to a naive T cells by an APC, chances are good that a naive T cells will not be "stimulated" or "activated".
Depends on what you mean by activation.
When you have the naive T cell, the APC and the relevant antigen present (in vitro): If measuring activation by secretion of IFN-g, it takes 24-48 hours to see a measurable response (by CBA, ELISA or ELISpot). If measuring CD107a expression, you would see a response within 6 hours. If looking at T cell proliferation as a measure of activation, it takes 5-6 days.
In any case, the time required for activation would depend on a multitude of factors including the reactivity of the donor T cells, immunogenicity of the antigen ( strong antigens need less time), the nature of the antigen (peptides take less time compared to full length proteins), the antigen presenting capability of the APCs and so on.
Hi, I am agree with Julie Joseph. Additionally, I’d like to mention T-cells activation by superantigen. In this case, a polyclonal activation of T-cells takes place, and a healthy ( in the morning) person may become seriously ill, unconscious, with a temperature +41 °C, rush in the afternoon. The situation is well known as toxic shock syndrome after tampon use.
Depends on the pharmacological inducer used. My experience, with biosimilars of the granulocyte colony stimulating factor, is around 48 h
Hi Mushtak
Here is an article, it might help you:
T cell responses: naïve to memory and everything in between
Nathan D. Pennock, Jason T. White, [...], and Ross M. Kedl.
Adv Physiol Educ. 2013 Dec; 37(4): 273–283.
(A T cell response typically peaks ∼7–15 days after initial antigen stimulant ion) .
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