Young, migrating neurons are often seen riding or in close proximity to the vasculature. I am looking for a protocol to stain the vasculature that will not interfere with DCX/BrdU and cytoplasmic labeling. Any recommendations are appreciated.
CD31 for endothelium and alpha-smooth muscle actin for tunica media (smooth muscle cells)
Do you plan chromogen or fluorescent labeling?
Thanks, I'm most likely going to use fluorescent labeling.
I did a Glucose stain that worked once. Not sure if that helps. Guess it depends on what you are looking to quantify?
Hi Janice, a recent study found that in neurogenic regions, the vasculature is slightly permeable, allowing for small molecules to diffuse from the bloodstream. Our lab wants to quantify the number of capillary associated new neurons expressing DCX, and BrdU/Hu. Is your glucose stain protocol explained in your VEGF publication? I'll look into this. Thanks!
It seems to me CD31 and BrdU on Fluo mode will do the job.
Also serial sections. Do you plan FFPE or cryo?
Using an antibody to von Willebrand factor (most are polyclonal) has an advantage of cytoplasmic staining of endothelial cells. If you want to show proliferating microvessels then use CD105 (endoglin, part of the receptor complex for TGF-beta) works well. Good luck with your project.
Maria and Ivan, thank you for the suggestions. I'll look into this.
- Badges
- Science topic
- Similar topics
- Methodology
- Laboratory
- Histological Techniques
- Histocytochemistry