I have to stimulate my cells with IFN lambda or IFN beta and compare the levels of stimulation.
I need to stimulate my cells at the same concentration expressed in untis/ml. Also because the IFN-beta that I have, has been resuspended at a concentration expressed in units/ml.
Does anyone knows the conversion factor?
Thank you
The commonly accepted definition is that 1 unit is equal to the ED50 in a given bioassay. However, varying assay conditions can affect the ED50. Therefore, to follow a protocol given in units or to compare a commercially available product sold in units, one must verify how the unit was defined. It is strongly recommended that a dose response be done when beginning experiments, or with new lots . It is recommended to start with the midpoint of the ED50 range as the midpoint in the titration, and also running samples at 5, 10, 20, or 50 fold increments above and below this point.
Hope this helps
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