A kingdom of eukaryotic, heterotrophic organisms that live parasitically as saprobes, including MUSHROOMS; YEASTS; smuts, molds, etc. They... | Contact experts in Fungi to get answers
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Questions related to Fungi
I am working with A.niger in small bioreactors (0.4L) to produce citric acid. For this purpose, the fungi must to be in pellet form. I reached this goal, however I have some biomass attached on...
17 September 2021 7,304 1 View
I need to this preparation for the culturing of the roots in the laboratory so that infection of the Meloidogyne could be develop on this. A proper protocol of this will be appreciated. kindly help
11 September 2021 5,818 3 View
Hi! I am looking to make a medium for culturing fungi. I plan to make DCPA (Andrews and Pitt, 1986 Selective medium for isolation of Fusarium species and demat... ) and I am wondering if I can use...
07 September 2021 8,451 7 View
In LAB fermentation medium, potassium phosphate dibasic is used as one of the nutrient component. However, pH is also adjusted at various levels. In this case, what is the use of potassium...
07 September 2021 7,866 3 View
Hello, I'm trying to select among different peptones from different sources such as potatoes, meat, milk solids etc. for C. Elegans growth medium. What difference do they make? Any suggestions...
01 September 2021 6,580 4 View
I need to build a multiple sequence alignment for my project, as I'm designing a PCR assay. I'm confused as to whether I should build the alignment using my PCR product or with the sequence I used...
20 August 2021 7,769 5 View
I recently isolated some filamentous fungi from soil and sediment samples. I have been trying to identify them using macroscopic features as well as the microscope whereby I prepare a wet mount of...
16 August 2021 2,098 25 View
Hi, I am trying to isolate RNA from Chilli matured leaves (Capsicum annuum) with RNAiso plus reagent (TAKARA) method. The 260/280 ratios are in the range of 1.90 to 2.05, and 230/260 are in the...
10 August 2021 5,169 4 View
Hello Guys, I'm working on an object detection project to recognize different plant diseases in the leaves and I would like to increase my dataset. Does someone know an open-source dataset with...
07 August 2021 1,173 5 View
I'm starting to work on biofuel from microalgae but I have any idea to collect them with a simple méthod it's a work from my TFC
13 July 2021 674 9 View
Dear all, I meet a big trouble in ChIP assay. I prepared the cell lysis buffer/shearing buffer containing 1%SDS which helps sonication well. However, 1%SDS damages the epitope of target protein...
25 June 2021 3,726 1 View
I am looking for a standard extraction procedure for herbal samples or Herbal powders by HPLC- FLD Detection? Thank you , Srinivasu K
24 June 2021 853 6 View
We wanted to test the effects of Yeast concentration in lipase production. Can you suggest any methods that could help us? Thank you very much!
22 June 2021 4,389 3 View
Hello, I tried to submit FASTA result of the ITs gene (Fungi), But the results don't include 100% matching, and the descriptions par has multiple, completely different probabilities.
14 June 2021 7,380 3 View
I am having problem with the determination of Peroxidase in Native PAGE. I am using stacking gel and saperating gel 4% and 12%. For staining, i am using Guaicol and H2O2 but still getting no...
14 June 2021 8,851 4 View
There are several studies on the method of contaminating soil with heavy metals. Which is better for contaminating the soil with heavy metals by adding dissolved concentrations in a known...
18 May 2021 8,234 3 View
Hello fellow researchers. I am confronted to a filamentous fungi subculture problem: Because of the lockdown, I could not properly store my filamentous fungi. When I got back to work, I could...
25 April 2021 3,334 3 View
When a Maize plant is given normal condition for growth development, stand & establishment, it grows without tillering. But, when it get infected by any infestans for eg, Stem borer or FAW...
16 April 2021 7,141 3 View
while isolating basidiomyctes from wood , some ascomycetes (trichoderma, Aspergillus) thrive more faster and contaminate the plates, i'm looking for a method or a chemical that slow or inhit the...
15 April 2021 7,653 6 View
Currently I am working on siderophore producing bacteria and I am unable to get the CAS media for the same. Please inform me.
01 April 2021 3,118 5 View
I got a mold in sterile milli Q water. It has white in color. I am curious, what it could be and how it can grow in conditions without anything with just a pure water supply.
26 March 2021 4,279 8 View
I have been working on the early detection of a gastric cancer biomarker in gastric disease patients. And facing difficulties in protein extraction from serum samples.
16 March 2021 7,280 0 View
I have isolated some marine fungi and want to analyse their metabolites. So I want to some simple metabolite production methods, apart from using broths (if there is any)
11 March 2021 2,668 2 View
Incompletely cross-linked pdms are difficult to remove.
02 March 2021 1,099 3 View