A kingdom of eukaryotic, heterotrophic organisms that live parasitically as saprobes, including MUSHROOMS; YEASTS; smuts, molds, etc. They... | Contact experts in Fungi to get answers
2,584 views 4,196 posts
Questions related to Fungi
I am investigating the levels of COL4A1 protein in a patient fibroblast cell line. Based on RNA analysis, I know the patient has a heterozygous variant resulting in aberrant RNA splicing and...
13 April 2025 6,985 0 View
How and when the pH of a green/photosynthesized nanoparticle be adjusted to 7?
10 April 2025 8,988 2 View
Which DNA barcode is best for identifying native plants? I'm currently using ITS and trnL, but I'm not getting enough resolution at the species level. For one plant, DNA amplified with both ITS...
03 April 2025 1,523 4 View
Hello everyone! I am currently working on the genomic integration in E. coli MG1655. I selected two attB sites to work with - attB lambda and attB 186. I have successfully integrated my gene via...
02 April 2025 2,068 0 View
My group is testing propolis extracts against a particular strain common in my region but when we used the broth microdilution method we saw dark green spots (similar to the propolis colour) at...
28 March 2025 4,309 1 View
Hi everyone, I'm trying to do a limiting dilution and isolate one clone from T24 cancer cells after a gene knockout. I followed these steps: First, used a hemocytometer to quantitate the...
25 March 2025 3,144 3 View
What is the best way and solution to dissolve the methanol extract of pomegranate peel in the treatment of oxidative stress in mice, as it is known that the methanol extract is toxic, and what are...
22 March 2025 1,417 5 View
How long should the implants be?
08 March 2025 2,791 1 View
so, we are looking at the main business or economic values after a successful implementation of resource efficiency and cleaner production to the mining sector.
25 February 2025 9,280 6 View
Hi, I have a major issue to discuss. There are two undergrads who are keeping our incubator open for many minutes because they are very slow in retrieving and putting back their plate. I am...
24 February 2025 4,112 0 View
What to do to improve the quality of oral health research?
21 February 2025 6,085 1 View
when I check the manual I found my problem as Any tab indicated with a padlock icon is a read only file that cannot be edited. can anyone help me to understand how to invert data into editable...
20 February 2025 5,684 0 View
I performed RNA isolation from infected wheat tissue using NucleoZOL, followed by agarose gel electrophoresis. Although I stained the gel with ethidium bromide (EtBr), I observed blue fluorescence...
17 February 2025 3,488 3 View
When I blended them separately, it formed a homogeneous solution. When I tried to blend them together it started forming a cloudy mix. Should I give them some temperature to make them a clear...
14 February 2025 3,599 1 View
I am having difficulty figuring out why my cluster density is consistently too high for my 16S sequencing runs on the MiSeq (v2 chemistry). I have been getting a cluster density between 1100-1300,...
14 February 2025 2,849 0 View
MOD inlays for Endodontically treated teeth have been known historically for high fracture rate clinically, particularly with wide cavities and thin remaining cusps, and with the conventional...
11 February 2025 7,815 3 View
I usually stain the ECM of blood vessels in a syngeneic glioblastoma GL261 model in C57BL6 mice using Collagen IV and/or Laminin without any issues. The signal is strong and colocalizes well with...
10 February 2025 5,083 0 View
Dear all, I am trying to isolate phages from environmental samples. I have performed the plaques assay and got good zone of lysis. But, when I go to next step to purify the phages using streaking...
09 February 2025 524 3 View
We have started culturing Leptospira and we noticed a greenish precipitate in the inoculum. We still don't have access to a 200X-400X magnification Dark field microscope, but could the precipitate...
08 February 2025 2,988 0 View
Please, I need to observe the MS2 phage in Transmission Electron Microscopy. The sample would be in a 1000 ppm NaCl solution before (1E7-8 PFU/mL approx) and after (0 PFU/mL?) an electrochemical...
07 February 2025 4,119 6 View
Hello, I tried a qPCR with OLG70/71 Primers for detecting Verticillium and somehow I get amplification for my negative control, that's a plant that wasn't inoculated and even my NCT shows...
03 February 2025 6,620 3 View
What is Space Management and What is Dental Crowding?
29 January 2025 4,797 1 View
Hello. I need some help regarding protein expression. My cloned gene is a thermostable amylase isolated from thermophilic bacterium and is cloned and expressed in Bl21 as well as pLysS strain....
26 January 2025 9,463 6 View
In my process, metal is melted in a crucible while the mould is simultaneously heated. Once the metal reaches the desired state, a steel billet is inserted into the mould and lowered. The stopper...
24 January 2025 4,494 1 View