Genome sequenced twice in the same technology are very different. How to explain?

Hi dear all, We are studying a fungal disease and we sequenced the same fungi twice, but with different extraction times. One 11 years ago and the another one this year. The strain was extracted...

04 October 2020 271 8 View

Which temperature should I use in Molecular dynamics (GROMACS) for a protein found in endoplasmatic reticulum?

Hi everyone, I'm trying to perform a MD simulation for a protein found in ER. In mdp file should I set: ref-temp= 300 K or 310K? gen-temp should be the same than ref-temp or 300...

22 June 2019 2,083 6 View

How to compare two RMSD plot (frame vs frame) from gromacs?

I have two proteins. One is wild-type and the other is a mutant. The RMSD plot (nm vs time) showed me mutant is far more unstable. But, when a plot the RMSD frame vs frame graphic it seems mutant...

28 May 2019 7,260 7 View

Has anyone used ALDEFluor Kit in bacteria cells?

I would like to know if the ALDEfluor kit can be optimized for use in bacterial cells? Has anyone used ALDEFluor Kit in bacteria cells?

29 October 2017 9,241 2 View

Maintenance of PFGE electrophoresis cell during long breaks (not in use for say 3 months)!?

What is the usual recommended procedure for maintenance of the PFGE electrophoresis cell during long breaks (not in use for 3 months): 1. Clear all the buffer/water from tubings, OR 2. Flush once...

26 April 2016 2,895 7 View

Alkylation of aliphatic nitrogen under aqueous alkali conditions result in formation of dialkylated product?

Will a hydroxyl group in the same aliphatic molecule also form ether bond?

20 April 2016 6,801 2 View

How can I extract a urease enzyme from leguminosae with its activity ready to use?

I desire quantify urea in plants extracts!

11 September 2015 9,878 3 View

Does anyone have experience with HPLC methods for soil organic phosphorus?

Hi Everyone We are trying to reproduce Espinosa et al (1999) method but so far it has been inconsistent in the retention time of the standards. We are still working in changing the mobile phase to...

19 January 2015 2,929 3 View

Is anyone using the NEB Lambda ladder PFG Marker?

01 November 2014 5,626 11 View

What are the prospectives of additives for synergism along with antibiotics against antibiotic resistant bacteria?

Synergism along with antibiotics against antibiotic resistant bacteria!

14 September 2014 8,747 8 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to upload pdf of my articles published?

How to upload pdf of my articles published?

03 March 2021 2,183 3 View

No title

02 March 2021 932 1 View

APA 7th In-Text citations with two references with three authors with the same surname, but four different author?

I have an issue with APA 7th in-text citation. II have two papers where the first three authors are the same, and the fourth is different. I am using the Mendeley desktop app, and automatically it...

02 March 2021 790 3 View

Where can I find life cycle inventory or assessment of non woven materials?

Dear network, I am looking for the LCA or LCI or at least the energy balances of the non woven fabrics process for my master project, in specific for natural fibers, yet I have found...

02 March 2021 9,662 1 View

Would adding books published in Lambert Publishers increase my RG impact?

There are some books published in LAP. Shall I add it to the RG?

02 March 2021 2,753 1 View

How Download/Extract citations of 1 given year ALL my publications in Google Scholar?

If you google this you find a lot of smart guys explaining you how to download 1 year of citations of 1 single paper of yours (or anyone else). I mean 1 year of ALL my papers. or ... ALL years or...

01 March 2021 1,121 9 View

How can I edit suggested publications?

Hi there! I regularly receive suggestion about papers and authors that are totally out of my area. Does anybody know how I can fix this? Thank you!

01 March 2021 9,574 4 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Any research paper call for publication related to management or finance or economics?

It should be standard journal (Indexed)

01 March 2021 8,379 1 View