Hi,
Did anyone measure calprotectin in mice feces using ELISA? if so, did you dilute the feces supernatant?
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30 May 2024 7,042 0 View
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19 October 2023 9,190 4 View
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14 October 2023 6,462 2 View
I usually treat the cells for maximum 3 days, what i usually do is that i seed for 24 hrs (180uL cell suspension in each well (96-well plate) and then on the next day i add the treatment on top of...
06 October 2023 5,719 0 View
post liver transplantation care , health outcomes
07 June 2023 4,381 0 View
I want deep understand how to write Literature Review as professional research
04 May 2023 6,639 4 View
if the LAB incubated anaerobically , why do we use catalase to inhibit H2O2 ? hydrogen peroxide produced when the oxygen is available so why do we need to inhibit it in the absence of oxygen ?
27 February 2023 1,496 1 View
Hello. We are working on LAB bacteriocins to detect the antimicrobial activities against pathogenic bacteria. We grew LAB on MRS broth and obtained CFS, it showed inhibition zone , but what is the...
12 February 2023 4,362 3 View
I need to measure the chemical oxygen demand for cooking oil that I am using for Anaerobic digestion. I tried high range, high range plus, and low ranges COD kits, and still no luck. After...
08 October 2022 1,730 0 View
I looked at biomethanation, H2 supplemention and a two-phase system, but I was hoping to look at other technologies if they existed. Any recommendations would be appreciated.
08 October 2022 9,451 4 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,081 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,156 4 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,706 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 761 1 View
I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...
31 July 2024 1,121 4 View
I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...
31 July 2024 9,186 0 View
I am developing an ELISA kit for rotavirus A (G1P8 and G1P6). Does anyone know the weight (ng) of these two strains? Or to help me find their weight?
29 July 2024 3,138 2 View
We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?
29 July 2024 8,402 4 View