Pyrimidine proportion in bacteria and yeasts?

How much Yeast should we add to fermentation media?

I have prepared a liquid broth containing Yeast (S. Cerevisiae) that i need to add from it to fermentation media of lignocellulosic hydrolysate. I need to know based on what parameters do we add...

06 August 2024 662 1 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

Solvent recovery after extraction - Separation of Isopropanol + Water - how to do it best?

I want to make some yeast extractions with a protocol that uses Isopropanol and NaOH. So in the end i will have an azeotrope of Isopropanol and Water that comes from the cell and NaOH. Can you...

08 July 2024 9,201 2 View

Why my yeast colony PCR gel has one specific band , but the size is incorrect even when the plasmid DNA that is originally transformed is correct?

The plasmid that was transformed originally is correct (sequenced). I am expressing different gene targets from the yeast genome to optimize my protein. When I sequence the incorrect size, it...

01 July 2024 2,356 6 View

What are these small structures found when PBMC cells are isolated?

Image attached is at 20X. Cultured media for checking presence of bacteria and yeast and both tests were negative.

27 June 2024 7,473 4 View

How to perform the anaysis of taxonomic on raw reads from the mixed sample?

Hi, I am very new to Bioinformatics. Recently, I have the project that aims to perform taxonomic anlysis on raw reads from mixed samples taken from environment. For example, we perfromed NGS...

09 June 2024 6,537 3 View

Is it legally permissible to reuse algae biomass following the treatment of non-hazardous laboratory waste?

Hello, community, Could you please clarify whether current legislation permits the reuse of algae biomass after it has been used to treat non-hazardous decontaminated laboratory organic waste?...

05 June 2024 5,040 1 View

Yeast carbon base (YCB) + agar + BSA?

I'm planning to perform BSA degradation using YCB and agar, but I need a protocol. Does anyone have one they'd be willing to share?

02 June 2024 6,885 8 View

How can we extract DNA from yeast isolates without using any lysis buffer or lysis enzymes? Is there any manual protocol?

31 May 2024 548 4 View

How much glucose to add to LB media to prevent leaky expression?

I am already using BL21(DE3)pLysS cells but I am still getting leaky expression in pre-expression samples before IPTG is added. LB broth is tryptone, salt and yeast. Thanks :)

28 May 2024 2,820 2 View