Review and cite EARTH protocol, troubleshooting and other methodology information | Contact experts in EARTH to get answers
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Questions related to Earth
Hello everyone, I am studying the fascinating carbon(0) compounds and thought of exploring their bonding situation. I used ADF software for that purpose and did EDA-NOCV calculation for carbon(0)...
16 March 2025 3,160 3 View
Demonic plesiosaurs and pterosaurs battling in eternal darkness by Thomas Hawkins, 1840. What do you think about this picture?
12 March 2025 1,709 0 View
I am currently conducting research on abiotic salt stress in soybean (Glycine max) and I'm interested in learning about effective strategies for improving salt tolerance in this crop. Salt stress...
10 March 2025 8,299 3 View
Hi everyone, I'm a master's degree student and recently started working with endoplasmic reticulum stress (Er-stress). In the literature, I found that staining with Thioflavin-T (ThT) is one way...
09 March 2025 604 0 View
hello, I was reading the following paragraph in materials and methods: "To evaluate the trans-cleavage activity of Cas12f1 RNP with different guide RNAs, a reaction buffer was prepared by mixing...
05 March 2025 8,213 4 View
I have developed a method for 35 analytes in MRM. Through the method, I monitor 2 transitions for each analyte, making a total of 70 transitions. I have attempted to replicate the MRM method...
26 February 2025 9,901 3 View
I am a Co-Editor of the International Journal of Multicultural and Multireligious Understanding (IJMMU). I would like to inquire about the process and requirements for having our journal indexed...
25 February 2025 3,469 1 View
Hi All, We are attemting to differentiate the ReNcell CX Human Neural Progenitor Cell Line (https://www.sigmaaldrich.com/GB/en/product/mm/scc007) into neurons and astrocytes, as described in the...
25 February 2025 1,664 1 View
what are the limitations in ascertaining the precise time of death using current forensic and ecological techniques?
18 February 2025 9,384 1 View
Hello everyone, I would like to analyse MEF undergoing reprogramming into iPSCs by flow citometry. I saw that is really hard to dissociate them into single cells in particularly at the latest...
18 February 2025 9,245 1 View
Hi all, I am going to make silanol group (Si-OH) on the surface of silica by using piranha solution. However, H2O2 in my lab is stored for a long time and can decompose. I concern that if the...
11 February 2025 2,746 0 View
Good morning everyone! I´m trying for a long time to visualize nickel alloy dendrites in the scanning eletronic microscope without success. My samples are previously prepared (Vibrapolishing - 4...
24 January 2025 9,470 7 View
I have a drug of poor solubility, may i use PVA for the solubilization of drug to be given via IV. If can be give how much % can be used?
07 January 2025 3,518 3 View
I would like to measure the average molecular weight of PTFE or PFA polymer (Mw distribution is preferred). I'm sure that the GPC method does not work due to their insolubility into any solvents....
25 December 2024 9,563 0 View
Studies on the interactions between the central nervous, immunitary and endocrinological systems, the mind and behaviour, allows a theory of medical science to be formulated . The variability of...
12 December 2024 4,595 0 View
Could you please help me find a source for Detection and segmentation by Hybrid Deep Learning EfficientNetV2 and Vision Transformer (EffNetV2-ViT) Model for Breast Cancer using medical mammography...
10 December 2024 8,683 1 View
The ds-dDNAs were generated by annealing 10 μg of both forward and reverse oligonucleotides in a total volume of 20 μl at 95 °C for 5 min and allowing the reaction mixture to cool down at room...
30 November 2024 1,498 6 View
Hello everyone, I am performing a BSSE (Basis Set Superposition Error) calculation using the Counterpoise=2 option to calculate the hydrogen bonding energy between two small molecules with...
28 November 2024 3,510 2 View
Hello everyone, We have Genotyping-by-Sequencing (GBS) data for 117 Brassica Juncea population and Whole-Genome-Sequence (WGS) data for the two parental genotypes. Since the sequencing platforms...
20 November 2024 3,338 2 View
Is there a standard method for reference to determine Rhodamine dye concentration in wastewater using Spectro fluorometer ?
19 November 2024 2,354 3 View
Hello, I am trying to implement an attitude filter that can help to estimate roll/pitch angles from gyro/accelerometer/magnetometer sensor for a helicopter with several manoevres. The problem is...
18 November 2024 3,570 3 View
Hi everyone, My 260/280 ratios were higher and I suspect contamination of DNA or RNA. Is is DNA or RNA in the agarose gel? The shape looks moon shaped. The image looks like following:
07 November 2024 3,195 3 View
Can we predict the maximum compressive stress of material by knowing the thickness and maximum tensile stress of a bar? Really need some help, thanks for read
06 November 2024 4,719 1 View
Hi, I am preparing a monoclonal antibody sample for CE-SDS and using 2-ME as a reducing agent. Every once in a blue moon, a sample turns brown. Why would this occur?
05 November 2024 6,937 2 View