Yes, there are standard methods to determine Rhodamine dye concentration in wastewater using spectrofluorometry. Spectrofluorometric analysis is commonly used because of Rhodamine’s high fluorescence intensity. Standard methods involve preparing calibration curves using known concentrations of Rhodamine and measuring the fluorescence intensity at its excitation and emission wavelengths (typically 530 nm and 590 nm, respectively, for Rhodamine B). The calibration curve is then used to quantify the concentration in wastewater samples.

Note:

• Refer to ASTM D4129-05 for general guidelines on fluorescence analysis for water samples.

• Ensure proper sample preparation, including filtration to remove particulates, and maintain consistent pH conditions, as Rhodamine’s fluorescence intensity is pH-dependent.

• Calibration should account for potential interferences from other fluorescent substances in wastewater.

If you need a detailed method, the ASTM standards or publications on spectrofluorometric dye analysis are suitable references.

D Shekhar Rao

Yes, there are standard methods for determining the concentration of rhodamine in wastewater. Rhodamine, which is often used as a color indicator in chemical and environmental analyses, can be determined using various analytical techniques. One of the standard methods used for this purpose is fluorimetry.

Standard Methods for Determining Rhodamine in Wastewater:

• Hasan Altawil, ASTM D4129-05 seems to be colorimetric determination of total and organic carbon and not related to spectrofluorometry. Can you cross check?
• And regarding pH, should the pH of blank be made same as sample?