I have developed a method for 35 analytes in MRM. Through the method, I monitor 2 transitions for each analyte, making a total of 70 transitions.
I have attempted to replicate the MRM method using dynamic MRM, but unfortunately, I have observed a significant loss in sensitivity.
How is this possible? Are there parameters other than cycle time and EMV that I can adjust to improve sensitivity?
Thank you all for your help!
Were the cycle and dwell times changed? Decreasing the cycle time and increasing the dwell time usually gives higher sensitivity.
Also check DOI: 10.5478/MSL.2010.1.1.021
Yeghig Armoudjian Thank you for your help!
Yes, cycle times were changed but no increase in sensitivity was observed, just a better peak shape was obtained.
However, how can I decrease cycle time and at the same time increase dwell times?
Cycle time divided by the number of transitions is the dwell time, isn't it?
Hi Mariano, in MRM Transition, Sensitivity issue is the main problem most of the time. to make the method Rugged you have to make the chart by considering 1 parameter constant and vary the other parameters to check which parent to daughter ion change its intensity.
from this chart select the optimized parameters for getting intense peak. further also check the stability of your chemical in solution when you expose it to the high temperature to get it in ionic form in MS system. make sure your curtain plate is well cleaned.