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Questions related to Coating
Why does a coastal area have less variation in temperature than non- coastal area and why are coastal areas cool during the day?
26 April 2023 6,331 4 View
I have isolated yeast Mitochondria following a standard protocol. But while checking the purity of mitochondrial fraction by Western blotting, I am getting a histone 3 band in addition to a hsp60...
25 April 2023 2,688 3 View
I tried two different basis set to optimize my compound by DFT and HF method. I feel the rotational constant somewhat influences the symmetry of the molecular structure. But, I don't it is correct...
24 April 2023 4,509 4 View
I would to study apoptosis, but I haven't regular access flow cytometry to use Annexin V/PI staining. Could suggest me sensitive methods to investigate apoptosis?
20 April 2023 2,337 4 View
I have to assemble a rig for extracellular electrophysiology in mice brain stem. The target brain region is deep, and the mice will be head fixed during the recordings. I wonder how critical is...
17 April 2023 1,623 5 View
explain your idea
15 April 2023 8,714 3 View
I prepared thin PDMS film embedded with CsPbBr3 Perovskite quantum dot and doped Ag+ ions to PQDs inside PDMS. The XRD results showed that the peak intensity of (100) plane increased while...
14 April 2023 3,703 4 View
I am currently investigating what factors have a hysteresis effect on th polymer network. I suspect this can be explained by creep testing. By applying force to the material for different times...
14 April 2023 880 1 View
Isothermal curve of palladium..
13 April 2023 7,911 0 View
What happens if a wave beam is introduced into a waveguide line with some mis-alignment (Offset and or Tilt)? Does the wave beam behave like an optical ray and continue to carry the misalignment...
13 April 2023 789 1 View
I need to disperse metal Np in Liquid CO2. B4 trying to do in Liquid CO2, which medium would be best to try. Which have similar properties as Co2?
13 April 2023 935 0 View
Hello, We are trying to make a stock solution of PCN and having a difficult time getting into solution. Can anyone share experience? This will be given I.P. to mice.
13 April 2023 4,579 0 View
Dear ResearchGate community, I am currently conducting SILAC data analysis using the Perseus program. My aim is to obtain a list of proteins with significant and high H/L ratios. However, I have...
11 April 2023 8,953 0 View
I have multiple thin layers on my substrate and I want to use HMDS to improve the surface tension before using photoresist. The issue is that the developer solution will remove it and for the next...
11 April 2023 3,445 2 View
I am hoping to find some direction or options for the following issue: Issue started with only one research (out of 7) and spread to all researchers over time. When transfecting (have tried...
10 April 2023 179 3 View
is it really important to understand the technical details or judging without knowledge is fine?
10 April 2023 1,193 4 View
It seems that almost all humans tend to judge art easily and confidently. Without any solid and objective reference, how can they decide boldly and confidently about something that is to a great...
10 April 2023 5,149 15 View
Need to derive reflection matrices (with Fresnel coefficients) for air-non-magnetic and air-magnetic thin films.
09 April 2023 6,309 0 View
I'm trying to measure the ROS production from RAW 264.7 cells by using DCFH staining and analyst by flow cytrometry. The problem is the level of ROS production in control cells was high. I'm not...
06 April 2023 6,719 2 View
comment faire un test de l'efficacité de la moustiquaire
05 April 2023 3,428 2 View
I have platelet pellet in PBS in my lab, but I am not sure how to proceed with platelet adhesion assay using platelet pellet with minimal amount of PBS. Most of the protocol show platelet adhesion...
05 April 2023 9,535 1 View
In ANSYS Classic (Mechanical APDL), is it possible to ... > model a frame, like say, a portal frame with beam elements > apply vertical loads > switch on stress-stiffening SSTIF > do...
04 April 2023 8,653 0 View
Can we fix the direction of magnetic moments of atoms in a CASTEP geometry optimization? I want to fix the directions only, not their values. Thanks.
03 April 2023 5,337 7 View
I am looking for a non-slip and non-scratching material for curved and wet glass surfaces.
31 March 2023 8,565 3 View