I have generated a figure "Gene distribution by conservation" from 20 genomes of bacteria. Can someone explain how to interpret correctly?

I have generated a figure "Gene distribution by conservation" from 20 genomes of bacteria. Can anybody explain how to interpret it correctly? Thank you in advance

11 January 2021 3,346 2 View

Problem with PCR using plasmid as template?

Hi I am trying to do a PCR for a 1kb fragment using a plasmid of 11kb as a template. But unfortunately on the gel, the band is as large as the plasmid, that is 11kb. Would anyone know that that...

20 October 2020 7,631 3 View

How can I get access to the travaux preparatories of international conventions related to Intellectual Property Rights?

For instance, the 'Historical Archives' section of the 'Audio Visual Library of the International Law' website gives access to the negotiation documents related to various instruments of...

07 June 2020 3,135 3 View

How to do primary cell culture from tumor tissue and injection to mice?

Dear Karen, I am aiming to isolate primary cells from freshly resected tumor tissue from consented patient and injecting those cells to mice. We have failed two times doing so, because the cells...

15 January 2020 8,262 2 View

What are the miRNA profiling alternatives?

I am interested in looking at miRNA signature may be for 10-15miRNAs, but amount of input RNA is limited. qRTPCR is not realistic, since it can provide only one or two miRNA data. Nanostring and...

26 April 2019 9,862 3 View

Rod to spore shaped 'particles' in cell cultures, bacterial contamination?

Last week I had consecutive contaminations of some of my mammalian primary cells alone. It started with the cultures turning turbid after a day or two of incubation. I wiped down the entire...

11 February 2019 5,127 8 View

Is WatchPAT a home sleep monior?

Is WatchPAT from Itamar medical a home sleep monitor or sleep apnea monitor? Can it be used to generate the ground truth for sleep staging? Any reliable references of the use of the device as a...

11 December 2018 1,313 2 View

How to interface arduino to Visual basics 2015?

can someone suggest me any website for visual basics coding or else give tell me how can I learn it quickly

30 November 2018 3,130 1 View

What is the difference between Subject specific and Epoch specific clustering?

Many of the machine learning algorithms use k-means clustering to improve the clasification performance based on human physiological signals. However, while some studies uses a subject specific...

08 November 2018 2,624 1 View

What is the advantage of using statistical techniques before feature selection using wrapper method?

I have seen literature reporting the use of statistical techniques like Mann-Whitney U test and Cohen's D effect size to identify most suitable subset of features for a classifcation problem. But...

15 September 2018 6,687 2 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

No title

02 March 2021 3,060 3 View