Hi everyone, I'm a new PhD student working on DC metabolism and gonna use seahorse machine for the first time. I'm the first in my lab to use the machine so don't have much guidance. I'm wondering if purchasing individual blocking agents (olygomycin, FCCP, A&R) etc has any downsides compared to using the test kits from Seahorse Biosciences? Also is there a need to purchase the Seahorse media or can I just use normal RPMI? Thanks.
Merle Krebber
In the past, we have always ordered normal RPMI medium for Seahorse analyses. It is cheaper and the results are equally good. Make sure to fill the wells on the edge with equal amounts of medium-only, it will help you reduce variation and background.
Good luck!
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