ANOVA

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Questions related to ANOVA

I have a drug of poor solubility, may i use PEG4000 for the solubilization of drug to be given via IV. If can be give how much % can be used?

My drug is poorly soluble in DMSO. I tried 1%PEG4000 and it is dissolving, but i have doubt whether i can use it for IV. Because PEG4000 is a high molecular weight compond

08 January 2025 2,663 3 View

Getting vector sequence instead of cloned fragment sequence with PJET cloning vector ?

Hello All, I'm trying to clone a 1.5 kb fungal gene to a PJET cloning vector (Thermo scientific) that has 98% selection capability, and I'm also verifying the clone using the PCR, but every time...

31 December 2024 4,487 2 View

Attempting to fill a vacancy, how do leaders fill empty roles?

I am most interested in Educational Landscapes, Leadership, Hiring and Upward Mobility I am a doctoral student, counselor, teacher, baseball player and coach, as well as a snowboard instructor...

17 December 2024 4,248 1 View

Why is the protein expressed in prokaryotic expression less than the predicted amount?

The expected fusion protein is 70kda, but it is only 30kda. I used the pet-32a plasmid, and the tag of this plasmid is 17kda. What is the reason? There are not many rare codons, only one or two....

12 December 2024 4,696 1 View

Questions about human PBMC macrophage differentiation?

Hello, I just started to work on human PBMCs to differentiate them into M1 macrophages. I used JOVE's protocol for macrophage differentiation, but I didn't get the results I was expecting as...

02 December 2024 7,607 0 View

New replacement for the LKB Ultramicrotomy System Pyramitome, 11800?

I work at the departement of pathology, EM-lab and we are on the look out for a replacement/update for the old Pyramitome 11800 we still use. The one we have now is quite old, but the working...

28 November 2024 2,986 2 View

GSAS II showing error while exporting a CIF file?

GSAS II is exporting a CIF file but file size is 0 kb there is nothing in the file. Please guide me.

26 November 2024 343 4 View

Hello, can anyone please suggest me a proper method to lyse 3T3L1 adipocyte?

I have used RIPA buffer and centrifuge at 14000rpm for 15mins at 4 degrees but I don't see any fat layer dispersed on top of the supernatant. I am getting a clear supernatant (though I can see...

25 November 2024 8,496 5 View

How can i get this type of river Shapefile for south Bengal Rivers of West Bengal, India?

Because i want to know the sources of this type of river network to my study area

11 November 2024 7,217 4 View

Any available PDF/s or tables from WHO quality standards for wastewater and surface water?

Hello, I am in search of available PDF/s or the likes that has water quality standards for surface and wastewater from the World Health Organization (WHO). You do find a lot available for...

09 November 2024 2,720 2 View

How federal learning helps in Automated Detection and Classification of Road Anomalies?

Lets share your knowledge about federal learning influence in Automated Detection and Classification of Road Anomalies

06 November 2024 5,064 0 View

How can I isolate RNA from imbibed Arabidopsis seeds?

Does anyone have a more effective protocol for extracting total RNA from Arabidopsis imbibed seeds? I used a Plant Total RNA kit, but the yield was very low, and no bands were visible on the gel....

28 October 2024 3,202 2 View

Can you guys please tell me what are the problems you face with current automated lawn mowers and what are the your expectations?

I am planning to create a new automated lawn mower. For that, I need your ideas.

22 October 2024 4,992 1 View

What is the most effective buffer technique for optimizing the detection of high molecular weight proteins (500 kDa) in Western blot?

Protein Isolation and Gel Electrophoresis: Total protein from mammalian cells was isolated using RIPA buffer following the manufacturer’s protocol. The proteins were then run on a 3-8%...

09 October 2024 4,718 3 View

If a program doesn't solve a career problem, what should we use?

Times are moving fast nowadays, having many ways in the healthcare field, but none of them can be a better solution for patient records, should we use blockchain technology?

08 October 2024 9,721 0 View

Hyponatremia is a key complication in polyuria in PP? What preventative measures or treatments could reduce the risk of severe complication?

Electrolyte Imbalances in Polyuria

02 October 2024 9,344 2 View

How to Request Permission to Reuse Figure from an Article Published in Elsevier?

We intend to reuse a figure in a manuscript we are preparing for submission in another journal (different publisher) and we would like if someone could advise us on the process for obtaining this...

23 September 2024 1,199 1 View

How can I index a new journal in international databases? Where is it easiest to index new journals?

I am the editor of a new journal, the Journal of Agriculture and Rural Development Studies (JARDS) www.jards.ugal.ro, which I want to index in international databases. JARDS has an ISSN, and its...

13 September 2024 6,520 3 View

C2C12 cells diameter/size?

Hello everyone, Does anyone have a clear measurement of the size/diameter of C2C12 cells in suspension and when attached? Thank you for your help!

11 September 2024 1,718 0 View

Anyone else getting persistent ResearchGate popup about email permissions?

I keep adjusting these settings, but the popup is constantly there. It started just now, end of the morning Amsterdam time; was not the case yet about an hour ago. Very annoying.

05 August 2024 4,508 2 View

MC903 model of atopic dermatitis method?

Hi. Trying to set up the MC903 model of AD in BL/6J mice. I've tried daily application of up to 4nmol for 8 days and also every 3rd day application for 3 weeks and I'm struggling to get an...

05 August 2024 1,898 0 View

Has anyone a protocol to perform Biparental mating using the plasmid pMF230 (E. coli ) ?

Dear researchers, I am trying to tag a Pseudomonas synxantha, I got the plasmid of gfp pMF230, but looking at the articles used the same plasmid, I didn't find much information how they perform...

30 July 2024 2,307 8 View

What is a good flag antibody produced in rabbit for IP?

Hello, I'm looking at immunoprecipitating our flag tagged protein in a chondrocyte cell line derived from mouse. So I need recommendations for a good IP antibody for flag produced in rabbit that...

30 July 2024 6,826 3 View

I want to do western blot for a protein with 700 kDa high molecular weight. Any recommendation about the ladder and western blot condition?

I am using 6 % SDS-PAGE. I could only find a protein ladder with maximum band marker around 500 kDa.

17 July 2024 1,857 3 View

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