I have a total number of examined participants 642; the infected number is 61, and the Non-infected 581; any idea how to find the chi-square and p-value?
As Hamid Ghorbani explains, the answer depends on what specific null hypothesis you're evaluating. With a two-category variable as you have, if you opt for a chi-square test, you'll be using the chi-square goodness of fit test (not the chi-square test of independence). However, for a goodness of fit test to be appropriately evaluated, it's critical to know how to compute the expected cell frequencies.
If your null hypothesis was: no difference in rate of infected and non-infected (p1 = p2; which doesn't sound very plausible to me),
Then expected cell values are N/2 for infected and non-infected, respectively (where N is your total sample size).
However, if your null hypothesis is that the rate of infected cases is no different from some benchmark rate (e.g, p1 = .20, p2 = .80 if these represent prior empirical rates observed in other samples), then the expected cell frequencies are 0.20 * N for infected and 0.80 *N for non-infected, respectively.
So, there is no single answer as to what the resultant computation should be, without knowing your specific hypothesis.