Hi, there is white precipitate formed in my stock solution of BAP? Why is this happened and should I use the solution?
Hey! I aim to generate a transgenic knockin zebrafish line that mimetizes a genetic condtition that leads to a certain disease on human. To do so, I need to insert a codon for mutagenic aminoacid...
14 July 2024 6,240 0 View
I did research and the studies suggest controlling for the effects of covariates on all endogenous variables, including mediator variables, to improve model accuracy and reliability. I want to...
04 June 2024 1,914 3 View
I am looking for literature on how different interests (e.g. energy companies, governments, political parties, industries, business associatons, etc) use energy crises and concerns over energy...
31 January 2024 4,248 0 View
I'm using a transposon tol2 vector (pT2AL200R150G - 10.1534/genetics.106.060244) to create transgenic zebrafish line. We first inserted our gene of interest (GOI) into the BamHI site of the...
21 November 2023 9,618 1 View
Dear fellow researchers, I would like to know where I can find the editable/raw data for a set of points of a S-N curve for a high strenght steel, like a dual phase. I can only find the S-N...
19 November 2023 8,866 3 View
Matthaei (et al) writes that a common choice is Y_{ai} = 1/70; I've seen ranges from 1/60 to 1/100. Please could someone provide some intuition on the choice for Y_{ai} in Matthaei's design...
09 November 2023 5,741 1 View
I'm trying to determine the spacing between resonators for a planar bandpass combline filter, such that the even and odd mode impedances match those that I've calculated as part of the filter...
05 August 2023 3,005 3 View
I'm trying to design a tunable microstrip combline filter with its initial f0 at 2.45GHz, with my resonator's electrical length at 45 degrees to provide maximum tuning range. I'm having problems...
02 August 2023 8,614 5 View
I have some shotgun data (MS spectra) that I analyzed in Proteome discoverer implementing the Minora node (which I understand is used to do label-free quantification). However, I have read so many...
04 June 2023 4,332 1 View
We are exploring possibilities to adapt our stereo microscope due to the high cost of fluorescence stereomicroscopes. I recently came across this product...
16 May 2023 9,575 2 View
How do I calculate the stock solution to apply 100 uM MeJA for 1000 ml of plant nutrient medium ?
01 August 2024 723 0 View
I have been harvesting monocyte-derived macrophages from buffalo and cattle fresh whole blood using the same protocol for years and getting good macrophage cultures. Below is the protocol that I...
23 July 2024 4,074 2 View
I need to make a stock solution for radioisotopic incubations of freshwater sediments to study dark primary productivity but I have some confusion as to how I calculate the concentration of 14C...
15 July 2024 5,898 0 View
Hi All: I need to precipitate extracellular protein from supernatant. A bit confused with ammonium sulfate calculation. Should I be adding: 1. 65g of ammonium sulfate in 100 ml of...
09 July 2024 5,508 0 View
I synthesize silver nanoparticles ,and reserve it in water. Then, when I sonicate it some minutes or longer ,it couldn't be dispersed, most of them aggregate .Otherwise ,when I sonicate several...
09 July 2024 7,335 0 View
I am working on an acetyltransferase that is highly unstable. Its pI is 6.65, and its molecular weight is around 18 kDa. The protein elutes at 1M imidazole and begins to precipitate immediately...
19 June 2024 2,590 0 View
methylene blue stock solution
19 June 2024 10,030 3 View
The emerging markets have suffered from a state of stagflation. I hope you know your expectations according to your experiences regarding the implications for the stock markets,,,, risks and...
19 June 2024 4,410 3 View
Does someone know any precipitating agent to precipitate glucose from 10 % glucose solution? or to remove glucose from solution
13 June 2024 438 0 View
I saw the iPSC about a week after holding the stock (12 well and 3 stocks) and there were very few cells attached, and some cells were differentiated. I wonder what the problem is. The plate is...
12 June 2024 6,087 0 View