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Questions related from Yijie Daniel Deng
Several G- bacteria including Salmonella Typhimurium and E coli, have been engineered to treat cancers. One of the main things been found is that those bacteria can specifically colonize tumor...
31 October 2019 6,232 2 View
By knowing the mechanism and determining factors, we will know how to increase the transformation efficiency in microbes.
21 February 2017 2,913 4 View
We can easily get tons of 16S rRNA sequence data for microbial communities by next-gen sequencing techniques with primers (eg., V3 to V4). If we want to compare whether bacterial communities are...
19 October 2015 3,275 7 View
Many proteins are oxygen-sensitive. If I want a lot of those proteins, is there any solution to express and isolate those proteins without loss of their activity?
25 September 2015 8,729 4 View
Both aerobic and anaerobic bacteria need to have ETC to synthesize ATP. While aerobic bacteria use oxygen as the terminal electron acceptor, anaerobic bacteria use other substrates as the electron...
30 March 2015 2,293 12 View
Protein complexes perform essential functions in cells. Fatty acid synthase in human is a protein complex made of multiple proteins. RuBisCO is another protein complex. They are composed of...
28 March 2015 8,879 3 View
Bacteria like E. coli synthesize fatty acids through a repeat extension with malonyl-ACP and adding 2 carbon at a time. Once the fatty acid chain reaches a certain number, usually palmitoyl-CPA...
02 January 2015 2,188 3 View
GFP is a good reporter that can be tagged at the N- or C-termini, or even in the middle of a protein. What is the rationale for the different tagging strategies?
03 October 2014 8,546 9 View
I wonder some principles to tell the zeta potential of a material such as glass, cotton, paper or plastic. Are they positively or negatively charged in water? Why ?
18 September 2014 8,156 12 View
I want to design an artificial metabolic pathway in bacteria or just using responsible enzymes to produce certain products such as H2 or other biofuels. Where can I start with? Do you have some...
15 September 2014 1,244 5 View
A lot of mutation methods are PCR-based and the mutations in vectors are then transferred to host cells. Are there any ways to mutate a plasmid in the bacteria in vivo? I want to make random...
27 August 2014 4,496 5 View
Agar plates amended with substrates (eg, CMC, casein) can be used to screen for bacteria producing active enzymes such as proteinases and cellulases. Similarity, inhibition zone in Burkholder agar...
13 August 2014 555 8 View
I isolated Bacillus strains from soil by heat shock method. I want to make sure how many my isolates are belong to the species Bacillus subtilis. Do you know some specific targets for PCR test?...
01 August 2014 2,435 9 View
I am working with bacteriophage SPP1 to do transduction with Bacillus subtilis. I wonder how specific this SPP 1 phage to host bacteria? Does it work with any strains of B. subtilis or only some...
01 August 2014 8,785 3 View
I am working with a lot of bacterial isolates from the environment. I've already sequenced them and many are from different species. I try best to keep them pure and not mixed-up during my...
29 July 2014 2,626 8 View
We are always fighting with bacterial pathogens. However, the speed of discovering a new antibiotic is much slower than that of bacteria developing a resistance gene to that new antibiotic. What...
11 July 2014 9,798 19 View
In bacteria biofilm, a lot of bacteria become metabolically dormant while some are still actively growing. How do you separate them?
16 May 2014 233 12 View
I want to over-express one operon within a bacterium. The operon has about 10 genes, each 1 kb in size. How can I design the expression system?
12 May 2014 1,831 2 View
Some bacteria have very high species diversification with various phenotypes and genotypes. For example, Bacillus subtilis have a lot of subspecies and strains carrying different properties such...
05 May 2014 7,006 3 View
I knocked GFP into Bacillus at amyE locus but found results were interesting. Most successful transformants carrying Spectinomycin marker lose their ability to degrade starch. This was expected....
30 April 2014 289 5 View
We have been developing and using solar energy for a long time. What limits the large scale use of solar energy fuels? What are the technical problems we need to address before it can support a...
22 April 2014 2,228 33 View
Many research labs have both PhD students and postdocs. For principal investigators, what does a postdoc in your lab mean to you? What is your expectation of those postdocs?
31 March 2014 1,258 5 View
Usually, bacteria have multiple copies of 16S rRNA genes and also for some other functional genes. But most of the genes seem to exist in single copy in the chromosome. What is the reason for...
27 March 2014 1,662 26 View
Two integration methods are widely used: single and double crossover. Before I chose which one to use, I want to make sure the advantages and disadvantages of them.
25 March 2014 398 4 View
Inducible antibiotic resistance genes might give bacteria better fitness, while some bacteria constitutively express resistance genes. How are resistance genes regulated? Why some are inducible...
20 March 2014 7,409 31 View
For transformation of foreign DNA, E. coli prefer circular plasmid while Bacillus subtilis get higher transformation efficiency with linearized plasmid. What is the reason for that?
16 March 2014 6,760 17 View
CRISPR-Cas is a new gene editing technique with high target-specificity. It has successful applications in gene knock-out/in/down in many Eukaryota organisms including zebra fish, fruit fly, mouse...
04 March 2014 7,425 4 View
I want to use bacteria Vibrio to express an enzyme that need to be secreted into medium. What are some good vectors for that purpose?
03 February 2014 2,189 4 View
With new DNA sequencing techniques, we can sequence almost any kind of samples with microbes in and get a good idea of the structure and composition of the microbial communities. That is nothing...
04 December 2013 5,628 4 View
For experiments dealing with bacterial cultures or cell line, people usually grow a strain of bacteria or cell from one culture stock. If I grow a bacteria in 3 containers (eg, flasks) from the...
16 October 2013 5,430 21 View
I have a data set of pairwise interactions (present / absent) among 50 bacteria and I want to visualize their interactions in a network. What can I do? What program or software do you think I can use?
01 October 2013 1,276 13 View
Molecular tools allow us to measure the diversity and also abundance of some functional genes, such as nitrogen fixation genes(nif H), ammonia oxidation genes (amo B) etc. For the gene diversity...
05 September 2013 2,703 4 View
Why do microbes in our gut form a certain structure? Why do not they overgrow? Are there some limiting factors of their growth? Such as the source of C, N and P, oxygen availability, host immune...
28 August 2013 3,895 3 View
Although next-generation sequencing allows characterizing microbial communities on a very large data scale, it is still difficult to profile all rare microbial species from environmental samples....
26 July 2013 4,457 12 View
It seems that the smaller the size of an organism, the greater number of it in the environment. For example, bacteria is tiny, but the number of it is huge. Virus is even smaller, but its number...
19 July 2013 8,548 4 View
To get good PCR yield and reduce non-specific products, how much DNA template is generally used and how many cycles do you usually run for a PCR?
07 July 2013 2,366 87 View
I am measuring DNA concentration by using a fluorescent dye called Gelgreen on 96-well plates. Does the incubation time affect DNA measurement? Because I found some problems with the standard...
02 July 2013 8,655 9 View
Microbial ecologists often apply diversity indices from the macro world (eg., animals or plants) to the microbial world. If you get certain numbers from calculations, what do those indices really...
28 June 2013 10,056 25 View
Bacteria and fungi have been genetically modified to produce recombinant proteins, vaccines, pharmaceuticals, materials, fuels, etc. Finally, the reconstructed organisms will be put to large-scale...
22 June 2013 9,813 7 View
Microbes often form consortia performing material processing and cycling in the environment. Cooperation and interaction occur within those consortia. We can use microbial consortia to do work...
20 June 2013 4,087 5 View
It is often a problem when using 16S rRNA bacterial universal primer sets (eg., 341F/531R and 515F/806R) to amplify bacterial community DNA. I almost always get PCR products for negative...
16 June 2013 9,342 32 View
Bacteria have different sensitivity to their or others' autoinducers. When those chemical signals reach a certain centration, the population start to behave in a concerted way, by expressing some...
15 June 2013 2,351 1 View
I wonder why some labs are very productive and doing good science while others not. Do good labs have some patterns or structures that others can follow? To run a good research lab, what should we...
13 June 2013 1,091 49 View
The concentration dynamics of quorum sensing molecules such as AHL during bacterial growth; when do bacteria start to produce QS compounds? When is the QS compound consumed by bacteria? Does the...
05 June 2013 9,525 3 View
It is widely noticed that microbial consortia have the potential to enhance the degradation and conversion of lignocellulosic biomass. What are the underlying mechanisms?
03 June 2013 2,355 8 View
How do non-complexed cellulases recognize and bind to cellulose? How is cellulose hydrolysis by cellulases possible? What interactions occur between enzymes and the substrate? Those are some of...
23 May 2013 4,956 4 View
I want to test whether the AI-2 produced by bacteria A impact the growth of bacteria B. I want to extract AI-2 but remove proteins, sugars and others from culture broth that might also stimulate...
13 March 2013 2,135 8 View
Lignocellulose degradation is a key process in both the biofuel production and the carbon cycling in environment. For this reason, intensive studies in this field have been going on for long time....
28 January 2013 8,090 4 View
I want to count bacteria from biofilm with DNA probes and study bacterial interactions in biofilm.
28 December 2012 2,460 14 View
Gut microbiota play significant roles in human health. With the help of Next-gen sequencing, we know much about the compositions of bacteria in the gut but little is known about their activities....
01 January 1970 6,262 3 View
Many enzymes require coenzymes (NADH, NADPH, CoA, TPP, etc) to catalyze biochemical reactions, such as redox reactions. Does an Enzyme bind to substrate and coenzyme at the same time or bind to...
01 January 1970 1,484 5 View
Many important drugs are produced as secondary metabolites by microbes. Why are secondary metabolites mainly produced in the stationary phase where nutrients become scarce? How can we increase the...
01 January 1970 6,517 5 View
iGEM has a collection of many ribosomal binding sites (RBS) at varying strength. eg, BBa_B0030, B0031, B0032, and BBa-J61100-J61150. On the other hand, RBS calculator (...
01 January 1970 8,343 5 View
