What kind of tape can be used to fix the samples on an XRD machine?

I have a very thin sample, the thickness is around 1 mm. I want to do X-ray diffraction measurement on the thin side of the sample. What kind of tape can I use to fix the sample, and will not...

13 February 2021 4,153 3 View

How to deal with fMRI data with long task block (120s)?

Respected researchers, Recently, I have got a bunch of fMRI data collected from an experiment using a block design. However, it has a very long task block (lasting for 120 seconds) and containing...

11 December 2020 3,609 3 View

How to export the standard XRD spectra of titanium alloy under different X-ray wavelength?

Hello all, which software can be used to export the standard XRD spectra of titanium alloy under different X-ray wavelength? How to operate? Such as 0.11 angstrom or 0.6887 angstrom (These X-ray...

07 December 2020 6,433 2 View

How to confirm the crystal face in synchrotron XRD data (Debye-Scherrer rings) ?

28 April 2020 4,247 3 View

Hydrogel's pore size at nano scale?

Hi all! I am selecting hydrogel materials for my DNA application. The ideal hydrogel should have a pore size between several to tens of nm and the pore to be negatively charged. The purpose of...

19 February 2020 3,862 3 View

What's the differences between the two control structures of grid-forming converters? Which one is more promising?

As far as I know, the control structures of grid-forming converters can be classified into 2 categories: one is single-loop without current control loop; the other includes a voltage loop and an...

16 February 2020 6,169 3 View

Is the term "inertia" still be a meaningful concept in future power grids?

With more and more renewable power integrated into power grids, the proportion of synchronous generators significantly decreases. As many researchers recommended the renewable power to emulate the...

30 November 2019 4,849 26 View

What are the mechanisms by which many G- bacteria can target cancer cells?

Several G- bacteria including Salmonella Typhimurium and E coli, have been engineered to treat cancers. One of the main things been found is that those bacteria can specifically colonize tumor...

30 October 2019 6,123 2 View

How to interpret synergy score in SynergyFinder?

I am trying to test whether two drugs have synergy in inhibiting cancer cells using SynergyFinder. I would like to know how one should interpret the results after the syngergy score is...

29 October 2019 4,733 1 View

In western blot, what kind of problem will cause antibodies stain the top area of each sample strip?

According to the color or the loading buffer and the protein ladder, the pull-down progressed successfully. And I also get right signals of beta-actin (lower ones), but another primary antibody...

28 August 2019 3,702 7 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

Universal bacterial primers targeting a mRNA?

Hi, I'm looking for universal primers targeting a bacterial mRNA to quatify bacterial load by qPCR in a complexe mixture (eukaryote and prokaryote RNA). Ribosomal RNA being depleted, I absolutly...

22 February 2021 9,969 3 View

How we can measure Kemp % in wool without using of benzene?

Would you please let me to know about the methods for measurement of medullation and Kemp % on animal? My problem is that the wool sampling and sending to laboratory is not possible. So I would...

21 February 2021 7,260 4 View

Does anyone have any ideas why drug-resistant malaria is increasing in Southeast Asia even when those drugs are no longer used?

When dealing with bacterial toxins, there are three varieties of microbes that develop in your petri dish: toxic, resistant, and susceptible. Initially, there are only toxic and vulnerable...

21 February 2021 6,084 5 View

Could you please share your experience with me on the type of 96 wells plate for MIC determination: Treated Vs Untreated and Round-Vs Flat-bottomed?

I am working on the MIC determination of various antibacterial agents (both water-soluble and- insoluble) against S. aureus, MRSA, and S. pyogenes. Some guidelines recommend using round-bottomed...

17 February 2021 7,029 3 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View

Hello, how can i find a primer to identify Bacillus Sp. with PCR? Can someone help me with this?

I am looking for PCR primers for bacillus Sp.

15 February 2021 2,552 5 View

How much amount of DNA (ligation mix) should I use for transformation?

Hi Fellow Scientists! I am trying to clone my 300 bp insert into a 6 kb vector. I run a few different ligation reactions using (1:3 vector: insert molar ratios) 10, 20,30, and 40 ng/ul vector...

15 February 2021 9,563 6 View

Any natural extract (that is easily available) or any drug combination triple which i can use against gram negative?

I'm working on gram negative i want to use any natural extract (plant extract) or triple drug combination against its biofilm formation.

14 February 2021 1,403 3 View

Would using an asRNA for repressing transcription of a high expression Listeria monocytogenes non-coding RNA harbored in a plasmid be a viable option?

Hello all, I am interested in deleting a highly expressed non-coding RNA located on a Listeria monocytogenes plasmid. Currently, there are not any optimized gene deletion methods for Listeria...

09 February 2021 4,994 1 View