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Questions related from Yen Teng Tai
I have came across a paper where they observed a delay timing of a drug inhibitory effect and insulin stimulatory effect respectively on purine synthesis, and thus, they suspected that the...
10 October 2018 10,025 0 View
I have obtained the RNA-seq results, which is in an excel file with all the fpkm values. May I know how do I check the gene expression profiles? Is there any software for this or I gotta manually...
05 May 2018 3,449 9 View
If overexpression of protein A can rescue the growth defect caused by mutation in protein B, can I consider protein A is working downstream of protein B? If yes, why is it so?
05 May 2017 7,616 24 View
Currently I am having problems in extracting membrane proteins (ammonium transporters), can anyone help? I used LDS in extraction and I did not heat the samples. Proteins in lane 1-4 seem like...
04 April 2017 875 20 View
I am preparing samples for BigDye sequencing. After PCR, I did ethanol precipitation to clean up the samples. In order to dry up the samples (after removing 70% ethanol), is it okay for me to dry...
10 October 2016 6,240 8 View
Currently I am working on a new protein in fission yeast. Based on the predicted function, it is involved in transcription. We found that when this gene is overexpressed, the cell growth is...
07 July 2016 8,827 1 View
One of the proteins in S.pombe that I am working on is tagged with FLAG, so can I still call this strain as wild type as it contains a fragment of foreign DNA? If no, what is the right term for it?
06 June 2016 8,204 3 View
I have digested my PCR product with NEB Not1 and Nde1 for 1hr. I have cut the band and purified the product. However I afraid the digestion is incomplete, can I re-digest them again? Is there any...
05 May 2016 2,758 17 View
Hi there. Anyone has protocol for TaKaRa PrimeSTAR mutagenesis basal kit in English? I have tried to search online but I can only get a Japanese version.
03 March 2016 4,976 6 View
May I know what is the maximum number of plasmids (with different origins of replication) can be taken up by a cell? What factors affect this? The maximum I have tried was two.
02 February 2016 4,425 4 View
May I know what are the characteristics of a BHR plasmid? Is this kind of plasmid occurs naturally or we can try to engineer a narrow host range plasmid to become one?
02 February 2016 7,268 0 View
I have came across a paper where they constructed a transformant named A. vinelandii rsmA::Smr/Spr. May I know what is the meaning of "::" ?
08 August 2015 9,555 2 View
May I know the difference between transformant and recombinant? I have identified a new gene from bacteria A and cloned into a plasmid. After that, I transformed the plasmid into bacteria B and...
05 May 2015 2,568 7 View
I have came across a term "acidic hydroxyl group", may I know under what condition the hydroxyl group will be acidic? I thought all hydroxyl group should be base?
05 May 2015 6,297 0 View
This is the phylogenetic tree constructed by a company using MEGAN 4.70.4, but I am not sure how to interpret the value. Can anyone explain to me?
11 November 2014 5,620 9 View
May I know what is the difference between all these proteobacteria? How is the classification done?
11 November 2014 3,661 1 View
The size of my gene is 1.7kb plus promoter is 800bp, so when I do PCR using plasmid primers, I should get 2300bp. However, I only can get 1500bp after I ran gel. I have screened through many...
07 July 2014 4,429 20 View
I have done a soil analysis on limestone soil sample. However, I do not know how to interpret the data. How can I know this value is consider as high or low? Where can I find the standard value...
05 May 2014 6,570 3 View
I am using genomic DNA from soil samples. I have sonicated the DNA template, blunt the end with enzymes, and then self-ligated them. Then I proceeded with PCR but the result is that my products...
03 March 2014 3,684 3 View
I have sent my soil sample for analysis. One of the results obtained shows hydrolytic acidity is not detected. May I know what is the meaning of hydrolysis acidity?
03 March 2014 8,899 5 View
Currently I have designed a few sets of primers, most of them the Tm is 69-79C. So is this okay since some of the Ta are higher than the temperature of polymerase.
02 February 2014 1,519 6 View
I have sent my soil sample for amplicon sequencing, the primers are targeted on bacteria V3-V5 region. However, the result that I obtained contains other things such as animalia, archae and...
12 December 2013 4,312 6 View
May I know if it is true that gene with any sequence also can be inserted into pGEM-T Easy? Since it has AT-overhang, so will this affect the type of gene inserted?
10 October 2013 3,006 16 View
After I did blue-white screening, I picked the white colonies and subculture on LB/Amp plate. They can grow well but when I subculture again on new plate, they cannot grow. May I know the reason?...
10 October 2013 3,078 2 View
Is there any special feature?
10 October 2013 8,735 2 View
...
07 July 2013 7,450 7 View
I have done a lots of colony PCR recently but all of them do not show band after amplification, so I wonder is it because I didn't subculture them after transformation (keep for quite long). May I...
07 July 2013 8,325 13 View
My bacteria has been left on plates for 2 months, can they still be revived, or not?
07 July 2013 2,949 2 View
the sample using gel electrophoresis. After that, I sonicate the sample but when I want to cut gel
04 April 2013 5,684 11 View
I am extracting DNA from soil sample based on Zhou's conventional method but I am facing problems with the humic acids. The humic acids will decrease the purity of DNA and thus, inhibits the...
04 April 2013 5,271 4 View
volume of my samples (~30uL) into the gel in order to see the band although the concentration of DNA
04 April 2013 1,433 6 View
I found that one of my protein in S.pombe degraded during nutrient starved condition. Therefore, I would like to check the degradation of this protein is caused by proteasome or not. Can I use...
01 January 1970 1,554 3 View
