Cellular Metabolism at ultra low temperatures?

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What is the best method to conserve the medicinal plant facing rapid decline in population ?

I have identified specific medicinal plant facing rapid decline in population due to population pressure, overutilization, unsustainable harvesting and limited distribution. What can be the best...

15 July 2024 6,599 2 View

Does anybody know if amino acids like GABA, BABA, proline, lysine, valine or any other non-aromatic amino acid are destroyed by UV light?

I am trying to eliminate infection from my plates and therefore, gave UV treatment after addition of amino acid (used for better growth). Can you please suggest if UV destroys amino acids or not,...

02 July 2024 2,922 1 View

What is the method of sampling for woody lianas in forest?

I want to study the population status of a particular liana in forest. I am not able to find sampling procedure to do the sampling in the study area.

12 June 2024 8,705 2 View

Beta cyclodextrin complex characterization methods?

Which is the best method for the characterization of beta cyclodextrin complex formation with guest molecule in solution form and in solid form ?

11 June 2024 6,719 0 View

How to get pure genomic DNA from CTAB method?

Hi, I have been trying to extract Plant genomic DNA via CTAB method and I am getting a 260/280 ratio ranging between 1.3 to 1.6. Any idea how to modify the protocol for fabaceae family plant...

03 June 2024 7,722 6 View

How to check a previously reported pair of primers?

Hi, I have few primers that are previously reported to amplify a conserved region. The ambiguity is that I want to be sure if they are correctly reported before ordering the same set of primers....

03 June 2024 4,167 3 View

In the mentioned image, what is the shiny particle? Is it also a microplastic particle?

Dear all, This is a microplastic image from the fish gut, and I am having difficulty identifying this shiny particle. I am guessing it may be a film particle. If someone knows, please guide...

27 May 2024 5,773 3 View

Low RFU for amplification curve in RT-qPCR?

I am performing some RT-qPCR with RNA transcripts (HIV-integrase) and as a control (RCAS transcripts). The amplification curve for my HIV int has low y-axis values. To rule out the possibility of...

11 April 2024 8,649 1 View

Drugs inhibiting pAkt but not pmTOR?

Hello All, Recently we found an interesting observations on cellular signalling events after some drug assays in breast cancer cell lines. The drug seems to be hitting pAkt, however no effect on...

14 March 2024 6,411 0 View

How to measure microplastic particle size under the microscope ?

Hello everyone I am a little confused about how to measure the microplastic particle size, I clicked the picture of microplastic, now would I need any specific software for that? kindly give me...

13 March 2024 2,801 4 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

What is the cycling of matter through ecosystems and how energy drives the cycling of matter in photosynthesis and cellular respiration?

02 August 2024 9,518 2 View

Is there a name for this type of function?

Let n∈ℕ, where the function f:A⊆ℝ𝑛→ℝ, 𝐴 is Borel, and (A,d) is a completely seperable space. What is the name of the functions where the graph of f in any (n+1)-dimensional box of A×ℝ is...

17 July 2024 258 1 View

How to prepare bacterial conditioned media to study the effect of bacterial secretome?

I would like to investigate the effect of bacterial secretome on cancer cells. How do you prepare conditioned media from bacteria?? conditions- time points - media I am looking for the best way...

17 July 2024 3,785 9 View

Can we preserve the protoplasts?

Can we preserve the isolated protoplasts for future experiments? Is it possible to preserve them via cryopreservation? If yes how long can we preserve them?

16 July 2024 9,576 1 View

How is the preparation to obtain secondary metabolic extracts?

Hi everyone! I am working with bacteria and fungal collected from marine sediments. I would to know the methodology since the preparation of biomass until the extraction and stored of secondary...

03 July 2024 6,177 3 View

List Plasmid suitable for Homology cassette in CRISPR-Cas9 protocol for Knock-In in mammalian cell line ?

Hello, I am working on creating a mutant endothelial cell line having Neomycin knock-in present, I have designed my homology arms of 1kb size having neomycin in between them, I would like to...

25 June 2024 1,197 0 View

Do narcissists have a proclivity for hacking cell phones and/or computers?

I have a client who was married to a narcissist (she diagnosed him). Though they are divorced now, she has thought a multitude of times that he ex-husband has hacked into her cell phone and...

04 June 2024 3,166 3 View

Error in COMSOL simulation when solving transfer function related to SnO2 gas sensor?

I'm trying to solve the transfer function to evaluate the SnO2 sensor performance in COMSOL multiphysics, thus I have developed a transfer function using interpolation functions and analytic...

03 June 2024 6,039 0 View