Sylvester Darvin Sandhaanam

28 Questions 34 Answers 0 Followers

Questions related from Sylvester Darvin Sandhaanam

3T3-L1 cells are growing slow in The DMEM/10%FBS media; any reason for that ?

3T3-L1 adipocytes

01 January 2019 3,278 4 View

Can any one give any simple protocol for annexin v pi staining for cell line flow cytometry analysis with or with out cell fixation ?

annexin v staining protocol adherent cells with cell fixation for flow flow cytometry

10 October 2017 465 2 View

Any place to Buy Hep3B cell line other than NCCS pune In India ?

Hep3B cell line

08 August 2017 6,356 0 View

Please do suggest any Liver control cell line For liver cancer ?

Liver control cell line liver cancer

02 February 2017 2,267 5 View

Hello Dear Scientists Please explain use of Chang liver cell line why we use? can we use for anticancer activity ?

Chang liver cell line why we use? can we use for anticancer activity ?

02 February 2017 832 0 View

Can any body tell about standard drug concentration for Cytotoxicity assay ?

Cytotoxicity cell lines

08 August 2016 2,843 2 View

Can i use "chang liver" cell line as control cell line for cancer cell line?

control cell

08 August 2016 1,765 0 View

Dear Friends Please help me to do autodocking in Mac OS, what are the sofware need and any installation and working procedure for that?

autodocking in Mac OS, Softwares in autodocking

07 July 2016 6,540 0 View

Can i use 5-fluorouracil as a control Drug for liver cancer cell lines ?

control Drug for liver cancer cell lines

07 July 2016 1,272 2 View

How to do dapi staining for Adherent cells? its same to PI staining for cell cycle assay please clarify ?

daps staining PI staining for cell cycle assay

07 July 2016 5,686 4 View

Can anyone tell me what is suitable control cell line for the liver cancer cell line HepG2 ?

control cell line for HepG2 cell line

05 May 2016 2,273 0 View

How to do the COMET ASSAY for cell line?

Complete PROTOCOL needed

04 April 2016 3,484 4 View

Can i use DMF instead of DMSO to Dissolve the drugs in cell culture please tell suggestions?

DMF /DMSO

11 November 2015 8,908 0 View

Can anyone help with plotting EC50 using Graphpad Prism for the MTT assay OD values?

I Have Control OD value and Treatment OD values

11 November 2015 4,442 1 View

Can anybody tell me standard protocol for MTT assay and its choosing vehicle and concentration and its calculation part and OD Nanometre?

How To dissolve Drug in DMSO and calculation part of it

10 October 2015 5,029 0 View

How to culture SH-SY5Y cells what are the assays perform from that?

SH-SY5Y culture

10 October 2015 4,450 0 View

Does anybody know any hepatocellular carcinoma cancer cell line control drug for treatment?

CONTROL DRUG FOR HEPG2 CELL LINE

09 September 2015 8,048 10 View

Can anybody tell how to dissolved the exracts or campound in DMSO and after that DMEM medium for cell culture ?

Concentration fixing 1mg to 100ug

04 April 2015 7,446 3 View

Anybody familiar with Anti-lipoapoptotic effect of plant extract on free fatty acids-induced HepG2 cells?

fatty acids-induced HepG2 cells and how prepare the extract in DMEM medium

04 April 2015 7,633 3 View

Is anyone familiar with performing MTT assay for Plant extracts?

MTT assay concentration fixing stock solution perpetration and require volume for test.

03 March 2015 4,260 3 View

How to select and fix the plant extracts or compound concentration to test for MTT assay cell culture?

MTT Assay

03 March 2015 9,340 2 View

Can any one suggest Protocol for isolation of proteins from cancer cell lines?

protein isolation

02 February 2015 6,253 3 View

Can anyone tell me complete protocol for fatty acid accumulation in HepG2 cell line?

Cell culture and FFA treatment in HepG2 cells using cultured cells were treated with various concentrations (0.05–0.5 mM) of long chain FFA with different degrees of saturation, including...

01 January 2015 6,354 3 View

Can anybody tell standard protocol for Preparing the DMEM media for cell culture step by step?

cell culturing media

12 December 2014 7,412 4 View

Can anybody tell me what are insect wing cell lines there are?

I am working on RNAi to suppress the wing development in mosquitoes.

10 October 2014 9,719 6 View

Can anybody please suggest some bio-assays for cancer cell lines?

I'm working on plant crude extracts. I want to check their anticancer activity for hepatocellular carcinoma. Please give suggestions for cell lines and bio-assays for standardization.

10 October 2014 8,109 5 View

Can anybody tell me if there is another substance, aside from Matrigel, for dish coating like Collagen ?

Please can you tell me the ratio of that substance.

09 September 2014 259 10 View

Did you established cell line, and what basis you are selecting the plants ?

stem cells

01 January 1970 345 2 View