3T3-L1 cells are growing slow in The DMEM/10%FBS media; any reason for that ?

3T3-L1 adipocytes

31 December 2018 3,180 4 View

Can any one give any simple protocol for annexin v pi staining for cell line flow cytometry analysis with or with out cell fixation ?

annexin v staining protocol adherent cells with cell fixation for flow flow cytometry

09 October 2017 393 2 View

Any place to Buy Hep3B cell line other than NCCS pune In India ?

Hep3B cell line

07 August 2017 6,272 0 View

Hello Dear Scientists Please explain use of Chang liver cell line why we use? can we use for anticancer activity ?

Chang liver cell line why we use? can we use for anticancer activity ?

01 February 2017 763 0 View

Please do suggest any Liver control cell line For liver cancer ?

01 February 2017 2,201 5 View

Can any body tell about standard drug concentration for Cytotoxicity assay ?

07 August 2016 2,775 2 View

Can i use "chang liver" cell line as control cell line for cancer cell line?

07 August 2016 1,674 0 View

Dear Friends Please help me to do autodocking in Mac OS, what are the sofware need and any installation and working procedure for that?

autodocking in Mac OS, Softwares in autodocking

06 July 2016 6,466 0 View

How to do dapi staining for Adherent cells? its same to PI staining for cell cycle assay please clarify ?

06 July 2016 5,619 4 View

Can i use 5-fluorouracil as a control Drug for liver cancer cell lines ?

control Drug for liver cancer cell lines

06 July 2016 1,201 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

No title

02 March 2021 3,060 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View