3T3-L1 cells are growing slow in The DMEM/10%FBS media; any reason for that ?

3T3-L1 adipocytes

31 December 2018 3,180 4 View

Can any one give any simple protocol for annexin v pi staining for cell line flow cytometry analysis with or with out cell fixation ?

annexin v staining protocol adherent cells with cell fixation for flow flow cytometry

09 October 2017 393 2 View

Any place to Buy Hep3B cell line other than NCCS pune In India ?

Hep3B cell line

07 August 2017 6,272 0 View

Hello Dear Scientists Please explain use of Chang liver cell line why we use? can we use for anticancer activity ?

Chang liver cell line why we use? can we use for anticancer activity ?

01 February 2017 763 0 View

Please do suggest any Liver control cell line For liver cancer ?

01 February 2017 2,201 5 View

Can any body tell about standard drug concentration for Cytotoxicity assay ?

07 August 2016 2,775 2 View

Can i use "chang liver" cell line as control cell line for cancer cell line?

07 August 2016 1,674 0 View

Dear Friends Please help me to do autodocking in Mac OS, what are the sofware need and any installation and working procedure for that?

autodocking in Mac OS, Softwares in autodocking

06 July 2016 6,466 0 View

How to do dapi staining for Adherent cells? its same to PI staining for cell cycle assay please clarify ?

06 July 2016 5,619 4 View

Can i use 5-fluorouracil as a control Drug for liver cancer cell lines ?

control Drug for liver cancer cell lines

06 July 2016 1,201 2 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

How can I make stacked bar graph with scatter plot?

I want to make a stacked bar graph with showing each data value as dots. I tried with graph pad prism, version 6, but could not succeed. Does anyone have tried before making such graphs?

01 March 2021 9,383 1 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

LEDs on Vis Light photo degradation of MB water sol via N doped TiO2 in a mini flume?

Good day to all, I have no experience with light emitting diodes. I am building a glass mini flume to demonstrate the photo degradation of Methylene Blue in water running over self cleaning...

26 February 2021 4,026 5 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

CGA 300 Regulator for CO2 cylinder?

Has anyone ever tried to deliver CO2 with a CGA 300 regulator? I know that a CGA 320 regulator is standard for CO2. The issue is that I currently do not have any free CGA 320 regulators. I also...

25 February 2021 3,292 3 View

How to increase solubility of Poly acrylic acid in Dioxane solvent?

I am working with Polyacrylic acid (100.000Da) and instead of water, I need to use another solvent (dioxane) to dissolve PAA. However, because of the high molecular weight, it's quite hard to...

25 February 2021 2,225 5 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View