Hi

Id like to try creating some quite high concentration DNA origami solutions. Using PEG precipitation seems like a really good method but Im unsure if I should look for a pellet after the initial centrifuge step or if this is just the papers way of telling me to carefully discard the supernatant then resuspend the bottom of the ependorf used to precipitate - it seems like in a DNA origami fabrication of ~45nm scaffold in 25 microliters that any pelleted material would be very hard to see with the naked eye?

Any advice would be really appreciated. Thanks for your time.