Can I use a PCR purification column to remove TCEP from a sample of reduced, thiolated, ssDNA sequences?

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Mangal Singh

Yes you can, anything unbound to DNA will be removed in the wash step. See the nucleotide length that can efficiently bind to the column.

Steve Hawkins

I agree with the previous answer, TCEP is commonly used in the disruption process during column based RNA extraction and is removed during column washing, this will be the same with your PCR product.

Simon Butler

Thank you Mangal Singh and Steve Hawkins. Succinct and helpful.